{"id":838,"date":"2021-11-15T14:08:49","date_gmt":"2021-11-15T14:08:49","guid":{"rendered":"https:\/\/apomixis.eu\/personnel-detache\/"},"modified":"2026-02-27T14:28:53","modified_gmt":"2026-02-27T14:28:53","slug":"personnel-detache","status":"publish","type":"page","link":"https:\/\/apomixis.eu\/fr\/personnel-detache\/","title":{"rendered":"Personnel d\u00e9tach\u00e9"},"content":{"rendered":"

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Personnel d\u00e9tach\u00e9<\/h1>\n

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La p\u00e9riode de d\u00e9tachement est la p\u00e9riode pass\u00e9e par un membre du projet dans un institut h\u00e9bergeant, aux fins du projet. La personne d\u00e9tach\u00e9e est le membre h\u00e9berg\u00e9.<\/p>\n

[\/et_pb_text][et_pb_blurb use_icon=\u00a0\u00bbon\u00a0\u00bb font_icon=\u00a0\u00bbs||divi||400″ icon_color=\u00a0\u00bb#b78a31″ icon_placement=\u00a0\u00bbleft\u00a0\u00bb disabled_on=\u00a0\u00bbon|on|on\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb body_text_align=\u00a0\u00bbjustify\u00a0\u00bb body_text_color=\u00a0\u00bb#b78a31″ background_color=\u00a0\u00bb#fff2d8″ custom_padding=\u00a0\u00bb16px|24px|16px|24px|true|true\u00a0\u00bb custom_css_blurb_content=\u00a0\u00bbmax-width: 100%;\u00a0\u00bb border_radii=\u00a0\u00bbon|4px|4px|4px|4px\u00a0\u00bb border_width_all=\u00a0\u00bb1px\u00a0\u00bb border_color_all=\u00a0\u00bb#ffe1a5″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_css_after_last_edited=\u00a0\u00bbon|desktop\u00a0\u00bb]En raison des restrictions de voyage adopt\u00e9es pendant la pand\u00e9mie mondiale de COVID-19, tous les d\u00e9tachements du projet MAD ont \u00e9t\u00e9 report\u00e9s. En fonction de l\u2019\u00e9volution des consignes et du contexte, nous esp\u00e9rons pouvoir commencer les missions \u00e0 l\u2019international d’ici la fin 2021.[\/et_pb_blurb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00a0\u00bb1″ _builder_version=\u00a0\u00bb4.17.0″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb custom_padding=\u00a0\u00bb0px||||false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_row admin_label=\u00a0\u00bbYEAR\u00a0\u00bb _builder_version=\u00a0\u00bb4.17.0″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb4_4″ _builder_version=\u00a0\u00bb4.17.0″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_text admin_label=\u00a0\u00bb2025″ _builder_version=\u00a0\u00bb4.18.0″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

2025<\/h2>\n

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Dr. Fulvio Pupilli<\/h2>\n

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IICAR, Rosario, Argentine<\/h3>\n

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IICAR (Rosario, Argentine), du 11 septembre au 12 novembre 2025<\/h3>\n

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Dr. Viviana Echenique<\/h2>\n

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CERZOS, Bahia Blanca, Argentine<\/h3>\n

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Universit\u00e9 de P\u00e9rouse (Italie), du 1er octobre au 30 octobre 2025<\/h3>\n

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Photo: Viviana Echenique \u00e0 l’UNIPG<\/p>\n

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Juan Manuel Vega, doctorant<\/h2>\n

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IBBR, P\u00e9rouse, Italie<\/h3>\n

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IBBR (P\u00e9rouse, Italie), du 11 septembre au 12 novembre 2025<\/h3>\n

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Juan Manuel Vega, du groupe DREP de l’IICAR-CONICET (Rosario, Argentine), effectuera un d\u00e9tachement \u00e0 l’IBBR-CNR (P\u00e9rouse, Italie) au sein du groupe du Dr Fulvio Pupilli du 11 septembre au 12 novembre 2025. Au cours de ce s\u00e9jour, il travaillera \u00e0 l’am\u00e9lioration d’un assemblage pr\u00e9liminaire du g\u00e9nome de Paspalum simplex en r\u00e9alisant un \u00e9chafaudage bas\u00e9 sur le g\u00e9nome de r\u00e9f\u00e9rence de P. notatum. Il contribuera \u00e9galement \u00e0 l’annotation des g\u00e9nomes sexuels et apomictiques de P. simplex et \u00e0 l’identification et la caract\u00e9risation du locus de contr\u00f4le de l’apomixie (ACL) en suivant les strat\u00e9gies pr\u00e9c\u00e9demment d\u00e9velopp\u00e9es pour P. notatum. Des analyses comparatives seront men\u00e9es pour \u00e9valuer la structure du g\u00e9nome chez les esp\u00e8ces de Paspalum, et des ensembles de donn\u00e9es transcriptomiques provenant d’inflorescences seront utilis\u00e9s pour explorer l’expression g\u00e9nique dans la r\u00e9gion ACL. Les travaux seront men\u00e9s en collaboration avec Davide Perrone, Andrea Rubini et Fulvio Pupilli.<\/p>\n

Photo: de gauche \u00e0 droite, Fulvio Pupilli, Juan Manuel Vega & Davide Perrone<\/p>\n

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Dr. Juan Pablo A. Ortiz<\/h2>\n

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IICAR, Rosario, Argentine<\/h3>\n

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IRD (Montpellier, France), du 1er septembre 2025 au 30 octobre 2025<\/h3>\n

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Dans le cadre des WP3 et 4, Juan Pablo A. Ortiz, de l’IICAR-CONICET (Argentine), a s\u00e9journ\u00e9 \u00e0 l’IRD, \u00e0 Montpellier (France), du 1er septembre au 30 octobre 2025. Au cours de son s\u00e9jour, Juan Pablo a travaill\u00e9 sur l’analyse de la transmission de l’aposporie (un \u00e9l\u00e9ment cl\u00e9 de la reproduction apomictique) par les gam\u00e8tes femelles. \u00c0 partir des donn\u00e9es de s\u00e9gr\u00e9gation g\u00e9n\u00e9r\u00e9es \u00e0 partir d’une plante apomictique facultative hautement sexuelle, un mod\u00e8le d’h\u00e9r\u00e9dit\u00e9 de l’aposporie par les gam\u00e8tes femelles est en cours d’\u00e9laboration. Le plan de travail comprenait \u00e9galement la d\u00e9termination de la teneur en hormones (auxine, cytokinine, z\u00e9atine et acide jasmonique) dans les tissus reproducteurs des g\u00e9notypes sexuels et apomictiques de Paspalum notatum. Les analyses biochimiques ont \u00e9t\u00e9 r\u00e9alis\u00e9es \u00e0 l’IRD par Virginie Vaissayre \u00e0 l’aide de la m\u00e9thode UHPLC-MS\/MS. L’analyse de la concentration des hormones et de leur r\u00f4le biologique dans la reproduction apomictique sera men\u00e9e en collaboration avec le Dr Olivier Leblanc.<\/p>\n

Photo: Microscopie confocale \u00e0 l’IRD, Lorena Siena (gauche) et Juan Pablo Ortiz (droite)<\/p>\n

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Dr. Maricel Podio<\/h2>\n

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IICAR, Rosario, Argentine<\/h3>\n

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IRD (Montpellier, France), du 1er septembre au 31 octobre 2025<\/h3>\n

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Le Dr Maricel Podio, de l’IICAR (Zavalla, Argentine), a effectu\u00e9 un d\u00e9tachement de recherche \u00e0 l’IRD \u00e0 Montpellier, en France, du 1er septembre au 31 octobre 2025. Au cours de cette p\u00e9riode, elle a \u00e9tudi\u00e9 le paysage \u00e9pig\u00e9n\u00e9tique de Paspalum notatum<\/em> en utilisant la \u00a0technologie Oxford Nanopore de s\u00e9quen\u00e7age l’ADN g\u00e9nomique extrait de plantes t\u00e9traplo\u00efdes sexu\u00e9es et apomictiques. Afin d’initier la caract\u00e9risation de leurs \u00e9pig\u00e9nomes, des ovaires au stade de l’anth\u00e8se ont \u00e9t\u00e9 s\u00e9lectionn\u00e9s parmi des g\u00e9notypes pr\u00e9sentant diff\u00e9rents niveaux d’expression de l’aposporie. Le s\u00e9quen\u00e7age r\u00e9alis\u00e9 pendant le d\u00e9tachement permettra d’identifier et de quantifier les marques de m\u00e9thylation de l’ADN (5mC), qui seront ensuite compar\u00e9es aux profils de m\u00e9thylation de l’ADN des feuilles afin de mieux comprendre les sch\u00e9mas \u00e9pig\u00e9n\u00e9tiques associ\u00e9s au mode de reproduction. Toutes les exp\u00e9riences ont \u00e9t\u00e9 men\u00e9es en collaboration avec deux membres de l\u2019UMR DIADE, Julien Serret et C\u00e9dric Mariac.<\/p>\n

Photo: lab work at IRD<\/p>\n

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Sol Vega, doctorante<\/h2>\n

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IICAR, Rosario, Argentine<\/h3>\n

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IRD (Montpellier, France), du 1er septembre 2025 au 30 novembre 2025<\/h3>\n

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Mar\u00eda Sol Vega est accueillie \u00e0 l’IRD afin de mieux caract\u00e9riser le r\u00e9presseur de r\u00e9ponse \u00e0 l’auxine IAA16 chez l’esp\u00e8ce mod\u00e8le Arabidopsis thaliana<\/em>. L’orthologue de IAA16, IAA30, est r\u00e9gul\u00e9 \u00e0 la baisse dans les ovules apomictiques de Paspalum notatum par rapport aux ovules sexu\u00e9s. Les activit\u00e9s de recherche comprendront la d\u00e9termination du profil d’expression spatiale de l’IAA16 dans les tissus reproducteurs \u00e0 l’aide d’une construction portant le g\u00e8ne IAA16 fusionn\u00e9 avec le g\u00e8ne rapporteur Ruby (pIAA16:IAA16:Ruby). Des plantes doublement homozygotes seront \u00e9galement obtenues et \u00e9tudi\u00e9es, issues de croisements entre iaa16 et des lign\u00e9es d’identit\u00e9 cellulaire de gam\u00e9togen\u00e8se et d’embryogen\u00e8se (pWOX2:CENH3, pKNU, FGR7.0, pDRN), des lign\u00e9es de structuration de l’auxine (DR5, R2D2, PIN1, aux1) et des lign\u00e9es mutantes pour son cor\u00e9presseur TOPLESS et le g\u00e8ne TGS1 li\u00e9 \u00e0 l’apomixie. L’analyse ph\u00e9notypique sera r\u00e9alis\u00e9e \u00e0 l’aide de la cytoembryologie DIC et de l’analyse des lign\u00e9es de rapporteurs sp\u00e9cifiques \u00e0 un type cellulaire sous microscopie \u00e9pifluorescente et confocale. Enfin, Mar\u00eda Sol pr\u00e9sentera ses travaux lors de la 5e Conf\u00e9rence internationale sur l’apomixie, qui se tiendra \u00e0 Montpellier du 16 au 19 septembre. Le titre de sa pr\u00e9sentation est \u00ab Auxin response repressor IAA16<\/em> defective mutants show developmental alterations in female gametophytes and embryos in Arabidopsis thaliana<\/em> \u00bb.<\/p>\n

Photo: Maria Sol Vega (gauche) & Lorena Siena (right), IRD, Montpellier (France)<\/p>\n

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Dr. Jos\u00e9 Carballo<\/h2>\n

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CERZOS, Bahia Blanca, Argentine<\/h3>\n

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Universit\u00e9 de P\u00e9rouse (Italie), du 14 ao\u00fbt au 10 octobre 2025<\/h3>\n

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Dr. Juan Pablo Selva<\/h2>\n

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CERZOS, Bahia Blanca, Argentine<\/h3>\n

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NIAB (Royaume Uni), du 28 juillet 2025 au 27 Septembre 2025<\/h3>\n

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Dr. Lorena Siena<\/h2>\n

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IICAR, Rosario, Argentine<\/h3>\n

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IRD (Montpellier, France), du 29 juin 2025 au 30 novembre 2025<\/h3>\n

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Dans le cadre de ce d\u00e9tachement, Lorena Siena poursuivera la caract\u00e9risation fonctionnelle des g\u00e8nes TRIMETHYLGUANOSINE SYNTHASE1 (TGS1) et QUI-GON JINN (QGJ) au cours du d\u00e9veloppement reproductif d’Arabidopsis thaliana. Les activit\u00e9s seront men\u00e9es en collaboration avec les membres du groupe DIADE et comprendront : le s\u00e9quen\u00e7age d’ARN unicellulaire \u00e0 l’aide de la technologie ONT \u00e0 lecture longue pour analyser les diff\u00e9rences d’expression g\u00e9nique entre les plantes Col et tgs1 A. thaliana et la construction de plasmides con\u00e7us pour \u00e9tudier la fonction de TGS1 ; la caract\u00e9risation de l’identit\u00e9 cellulaire des m\u00e9gaspores extra-fonctionnelles observ\u00e9es dans les ovules qgj, en particulier \u00e0 l’aide du rapporteur pWOX2-CENH3-GFP ; et l’\u00e9tude des interactions potentielles entre QGJ et TGS1 en d\u00e9terminant le profil d’expression de QGJ (pQGJ-GUS) dans les ovules tgs1 et, r\u00e9ciproquement, celui de TGS1 (pTGS1:TGS1-GUS) dans les ovules qgj. De plus, Lorena participera \u00e0 la conf\u00e9rence internationale Apomixis2025, qui se tiendra du 16 au 19 septembre \u00e0 Montpellier, o\u00f9 elle a \u00e9t\u00e9 invit\u00e9e \u00e0 pr\u00e9senter ses travaux sur la caract\u00e9risation de QUI-GON JINN lors de la reproduction femelle chez les plantes.<\/p>\n

Photo: Lorena, Bambouseraie d’Anduze, France.<\/p>\n

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Dr. Luciana Delgado<\/h2>\n

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IICAR, Rosario, Argentine<\/h3>\n

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IRD (Montpellier, France), du 13 juin 2025 au 10 octobre 2025<\/h3>\n

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Dans le cadre du dernier d\u00e9tachement du WP6 du projet MAD, en collaboration avec Daphn\u00e9 Autran, nous ach\u00e8verons l’analyse quantitative des donn\u00e9es relatives \u00e0 la division et \u00e0 la croissance cellulaires dans les primordia ovulaires de Paspalum rufum<\/em>. Notre objectif est d’analyser la dynamique de la croissance et de la division cellulaires dans des contextes sexuels et apomictiques afin d’identifier les \u00e9v\u00e9nements diff\u00e9rentiels qui pr\u00e9disent la diff\u00e9renciation des initiales aposporiques dans les cellules entourant la lign\u00e9e germinale sexuelle chez une gramin\u00e9e apomictique. Cela permettra \u00e9galement de comprendre de mani\u00e8re exhaustive comment l’architecture et la forme de l’ovule influencent les processus de diff\u00e9renciation cellulaire pendant la formation de la lign\u00e9e germinale. Nous pr\u00e9senterons et discuterons \u00e9galement nos r\u00e9sultats lors de la V\u00e8me Conf\u00e9rence internationale sur l’apomixie<\/a>, qui se tiendra en septembre 2025 \u00e0 Montpellier, afin de c\u00e9l\u00e9brer la fin de ce projet fructueux.<\/p>\n

Photo: Luciana devant les serres de l’IRD<\/p>\n

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Dr. Michele Bellucci<\/h2>\n

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IBBR, P\u00e9rouze, Italie<\/h3>\n

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IICAR (Rosario, Argentine), du 29 avril 2025 au 30 mai 2025<\/h3>\n

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Le 29 avril 2025, le Dr. Michele Bellucci du CNR-IBBR, Perugia, Italie, a commenc\u00e9 un d\u00e9tachement \u00e0 l’IICAR jusqu’au 30 mai 2025. Il travaillera sur la transformation de Paspalum notatum par bombardement de particules, afin d’inhiber l’expression du g\u00e8ne PnIAA30 dans les plantes t\u00e9traplo\u00efdes sexu\u00e9es et apomictiques par RNAi. Le vecteur d’inhibition a \u00e9t\u00e9 assembl\u00e9 au CNR-IBBR et contient une r\u00e9gion du g\u00e8ne PnIAA30 clon\u00e9e en orientation oppos\u00e9e et le g\u00e8ne bar comme marqueur s\u00e9lectionnable. Nos observation ont montr\u00e9 que l\u2019expression de PnIAA30 est r\u00e9duite dans les ovules apomictiques par rapport aux ovules sexu\u00e9s. Notre hypoth\u00e8se est que l’inhibition de PnIAA30 (ainsi que certains de ses paralogues) pourrait d\u00e9clencher le d\u00e9veloppement d’embryons parth\u00e9nog\u00e9n\u00e9tiques chez les plantes sexu\u00e9es et\/ou augmenter le taux de parth\u00e9nogen\u00e8se chez les g\u00e9notypes apomictiques. Toutes les exp\u00e9riences seront r\u00e9alis\u00e9es avec le Dr Carolina M. Colono et le Dr Juan Pablo A. Ortiz.<\/p>\n

Photo: Carolina Colono, IICAR (gauche) & Michele Bellucci, IBBR (droite)<\/p>\n

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Dr. Fulvio Pupilli<\/h2>\n

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IBBR, P\u00e9rouse, Italie<\/h3>\n

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IICAR (Rosario, Argentine), du 5 avril 2025 au 28 mai 2025<\/h3>\n

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Dr. Alessandro Camperio<\/h2>\n

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University of Milan, Italy<\/p>\n

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CERZOS (Bahia Blanca, Argentine), du 17 mars 2025 au 15 avril 2025<\/h3>\n

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Au cours de ce d\u00e9tachement, j’ai \u00e9t\u00e9 form\u00e9 \u00e0 la r\u00e9alisation d’analyses bioinformatiques sur un ensemble de donn\u00e9es de s\u00e9quen\u00e7age d’ARN \u00e0 noyau unique g\u00e9n\u00e9r\u00e9es \u00e0 partir d’ovaires d’Oryza sativa <\/em>(\u00e9chantillonn\u00e9s \u00e0 diff\u00e9rents stades de d\u00e9veloppement) sous la supervision du Dr Jos\u00e9 Carballo. L’objectif \u00e9tait d’acqu\u00e9rir des connaissances en mati\u00e8re d’analyse transcriptomique \u00e0 noyau unique et d’\u00e9tudier l’expression de g\u00e8nes ARF s\u00e9lectionn\u00e9s, identifi\u00e9s chez d’autres esp\u00e8ces comme des g\u00e8nes putatifs impliqu\u00e9s dans l’aposporie et la diplosporie. Dans le cadre de ce travail, j’ai cherch\u00e9 \u00e0 g\u00e9n\u00e9rer des profils transcriptomiques \u00e0 haute r\u00e9solution capturant la dynamique d’expression g\u00e9n\u00e9tique des populations cellulaires gam\u00e9tophytes et sporophytes en \u00e9volution rapide pendant le d\u00e9veloppement des ovules. Ces donn\u00e9es transcriptomiques \u00e0 noyau unique seront utilis\u00e9es dans un but plus large, \u00e0 savoir les comparer avec d’autres ensembles de donn\u00e9es provenant d’esp\u00e8ces Paspalum<\/em> et Eragrostis<\/em>. Ce cadre comparatif a \u00e9t\u00e9 con\u00e7u pour mieux comprendre le paysage mol\u00e9culaire d\u00e9couvert dans le riz, en mettant particuli\u00e8rement l’accent sur la d\u00e9tection des g\u00e8nes exprim\u00e9s dans le nucelle et les tissus associ\u00e9s.<\/p>\n

Photo (de gauche \u00e0 droite): Viviana Echenique, Alessandro Mariani & Alessandro Camperio<\/p>\n

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Granpierro Marconi, Msc<\/h2>\n

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Universit\u00e9 de P\u00e9rouse, Italie<\/h3>\n

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Universit\u00e9 d’Al\u00e9la\u00efde (Australie), du 5 janvier 2025 au 21 mars 2025<\/h3>\n

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2024<\/h2>\n

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Dr. Alice Casati<\/h2>\n

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Universit\u00e9 de Milan, Italie<\/h3>\n

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Unidad de Gen\u00f3mica Avanzada (CINVESTAV, Mexique), du 25 novembre 2024 au 15 f\u00e9vrier 2025<\/h3>\n

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Dr. Alessandro Mariani<\/h2>\n

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Universit\u00e9 de P\u00e9rouse, Italie<\/h3>\n

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CERZOS, Bahia Blanca (Argentine), du 13 novembre 2024 au 26 mai 2025<\/h3>\n

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Au cours de ce d\u00e9tachement au CERZOS-CONICET \u00e0 Bah\u00eda Blanca, j’ai contribu\u00e9 au projet Mechanisms of Apomictic Development<\/em> (MAD) en \u00e9tudiant la r\u00e9gulation g\u00e9n\u00e9tique et \u00e9pig\u00e9n\u00e9tique de l’apomixie chez les plantes. Mes recherches ont port\u00e9 sur la cartographie des loci associ\u00e9s aux traits apomictiques par une analyse QTL dans des populations en s\u00e9gr\u00e9gation, pour identifier des g\u00e8nes candidats impliqu\u00e9s dans la diplosporie, la parth\u00e9nogen\u00e8se et le d\u00e9veloppement autonome de l’endosperme. J’ai effectu\u00e9 une analyse bioinformatique des donn\u00e9es de g\u00e9notypage et de s\u00e9quen\u00e7age \u00e0 haut d\u00e9bit, ainsi que des \u00e9tudes de structure et association des populations, afin de d\u00e9couvrir les r\u00e9gions g\u00e9nomiques li\u00e9es \u00e0 l’apomixie. La cytom\u00e9trie en flux a \u00e9t\u00e9 utilis\u00e9e pour \u00e9valuer les niveaux de plo\u00efdie et la taille du g\u00e9nome, ce qui a permis de classer ph\u00e9notypiquement les accessions. De plus, j’ai int\u00e9gr\u00e9 des donn\u00e9es sur la m\u00e9thylation de l’ADN et le s\u00e9quen\u00e7age de l’ARN afin de d\u00e9tecter les diff\u00e9rences \u00e9pig\u00e9n\u00e9tiques entre les individus apomictiques et sexu\u00e9s. Ces approches int\u00e9gratives ont am\u00e9lior\u00e9 notre compr\u00e9hension des bases mol\u00e9culaires de l’apomixie, conform\u00e9ment \u00e0 l’objectif du projet qui consiste \u00e0 exploiter ce trait pour am\u00e9liorer les cultures.<\/p>\n

Photo: Viviana Echenique (gauche) & Alessandro Mariani (droite) au CERZOS.<\/p>\n

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Giacomo Bongiorno, PhD student<\/h2>\n

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Universit\u00e9 de P\u00e9rouse, Italie<\/h3>\n

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CERZOS, Bahia Blanca (Argentine), du 20 septembre 2024 au 21 novembre 2024<\/h3>\n

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Au cours de ce d\u00e9tachement, j’ai r\u00e9alis\u00e9 une analyse comparative des variations de pr\u00e9sence-absence (PAV) chez Eragrostis curvula<\/em>, ce qui a conduit \u00e0 l’identification de 503 g\u00e8nes candidats pour l\u2019apomixie. Plusieurs de ces g\u00e8nes ont \u00e9t\u00e9 valid\u00e9e par RT-PCR. J’ai \u00e9galement proc\u00e9d\u00e9 \u00e0 l’appel de variants (SNP et InDels) en augmentant le nombre de g\u00e9notypes afin d’approfondir l’\u00e9tude de la variation g\u00e9nomique, des modes de reproduction et des niveaux de plo\u00efdie. L’arbre phylog\u00e9n\u00e9tique construit \u00e0 l’aide de ces variants pr\u00e9sente deux \u00a0branches principales, distinguant les g\u00e9notypes \u00e0 plo\u00efdie elev\u00e9e des g\u00e9notypes diplo\u00efdes et t\u00e9traplo\u00efdes. Cette analyse a permis de mieux comprendre les relations g\u00e9n\u00e9tiques entre les g\u00e9notypes de diff\u00e9rents niveaux de plo\u00efdie, mettant en lumi\u00e8re de nouveaux sc\u00e9narios li\u00e9s \u00e0 l’\u00e9volution du g\u00e9nome chez E. curvula<\/em>.<\/p>\n

Photo: Giacomo Bongiorno (gauche) et Jos\u00e9 Carballo (droite)<\/p>\n

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Ing. Caroline Michaud<\/h2>\n

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IRD, Montpellier, France<\/h3>\n

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IICAR, Rosario (Argentine), du 12 novembre 2024 au 13 d\u00e9cembre 2024<\/h3>\n

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Au cours de cette misison, j’ai contribu\u00e9 aux travaux de Luciana Delgado et Daphn\u00e9 Autran sur Paspalum rufum<\/em> en explorant les liens entre l’architecture du primordium de l’ovule et la plasticit\u00e9 du destin cellulaire menant \u00e0 l’aposporie. Pour d\u00e9terminer les sch\u00e9mas de prolif\u00e9ration cellulaire au cours du d\u00e9veloppement de l’ovule chez Paspalum rufum<\/em> sexu\u00e9 et apomictique aux stades pr\u00e9-m\u00e9iotiques, nous avons r\u00e9alis\u00e9 des exp\u00e9riences pour marquer les cellules en phase S du cycle cellulaire \u00e0 l’aide du kit Click-iT\u2122 EdU. Les ovaires ont \u00e9t\u00e9 diss\u00e9qu\u00e9s \u00e0 quatre stades pr\u00e9coces, trait\u00e9s pour l’incorporation et la d\u00e9tection de l’EdU, puis imag\u00e9s \u00e0 l’aide d’un microscope confocal ZEISS 880 \u00e0 l’Institut de biologie mol\u00e9culaire et cellulaire (IBR-CONICET) de Rosario. Ces images seront segment\u00e9es avec MorphographX (MGX) pour quantifier les taux de prolif\u00e9ration diff\u00e9rentiels entre les g\u00e9notypes. J’ai \u00e9galement collabor\u00e9 \u00e0 un projet d’Olivier Leblanc et Lorena Siena, visant \u00e0 construire des plasmides pour l’analyse fonctionnelle du g\u00e8ne TGS1 chez Arabidopsis thaliana<\/em>, dont la caract\u00e9risation est pr\u00e9vue \u00e0 Montpellier en 2025.<\/p>\n

Photos. Gauche haut : ma carte d\u2019enseignante de l’UNR! ; Gauche bas : le microscope confocal de l\u2019IBR, et; Droite: un diagramme des stades diss\u00e9qu\u00e9s de Paspalum rufum<\/em>.<\/p>\n

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Dr. Olivier Leblanc<\/h2>\n

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IRD, Montpellier, France<\/h3>\n

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IICAR, Rosario (Argentine), du 12 novembre 2024 au 13 d\u00e9cembre 2024<\/h3>\n

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Photo: traverser la Pampa de Rosario \u00e0 Bahia Blanca…<\/p>\n

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Dr. Tamunonengiye-ofori Lawson<\/h2>\n

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Cambridge, Royaume Uni, NIAB<\/h3>\n

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CERZOS (Argentine), du 31 octobre 2024 au\u00a0 15 d\u00e9cembre 2024<\/h3>\n

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Dr. Daphn\u00e9 Autran<\/h2>\n

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IRD, Montpellier, France<\/h3>\n

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IICAR, Rosario (Argentine), du 28 octobre 2024 au 31 d\u00e9cembre 2024<\/h3>\n

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Au cours de ce d\u00e9tachement, dans le cadre du WP6 du projet MAD, Daphn\u00e9 Autran (IRD) a collabor\u00e9 avec Luciana Delgado (IICAR-CONICET), avec l’aide de Caroline Michaud (IRD), pour d\u00e9crypter la morphogen\u00e8se de l’ovule chez l’esp\u00e8ce aposporique Paspalum rufum<\/em>. Pour d\u00e9terminer quels sont les premiers \u00e9v\u00e9nements cellulaires associ\u00e9s \u00e0 un programme de d\u00e9veloppement aposporique facultatif, nous avons profit\u00e9 de la p\u00e9riode de floraison de l’unique population de plantes de P. rufum<\/em> pr\u00e9sentant des phenotypes reproducteurs contrast\u00e9s et disponible \u00e0 l’IICAR, Zavalla, Rosario, Argentine. Ici, le processus de prolif\u00e9ration cellulaire a \u00e9t\u00e9 sp\u00e9cifiquement abord\u00e9, en caract\u00e9risant le nombre et la localisation des cellules en division pour des ovules vivants collect\u00e9s \u00e0 diff\u00e9rents stades de d\u00e9veloppement. Apr\u00e8s fixation, nettoyage et coloration, les \u00e9chantillons ont \u00e9t\u00e9 imag\u00e9s par microscopie confocale \u00e0 fluorescence (disponible \u00e0 l’IBR, Instituto de Biologia de Rosario). L’ensemble du jeu d’images 3D g\u00e9n\u00e9r\u00e9es sera ensuite analys\u00e9 dans le but de compl\u00e9ter les informations recueillies dans l’atlas de croissance cellulaire 3D des ovules de P. rufum<\/em> produit lors de pr\u00e9c\u00e9dents d\u00e9tachements.<\/p>\n

Image: Luciana Delgado (gauche) & Luciana Delgado et Daphn\u00e9 Autran (colonne droite)<\/p>\n

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Dr. Silvina Pessino<\/h2>\n

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IICAR, Rosario, Argentine<\/h3>\n

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Universit\u00e9 de Milan (Italie), du 2 octobre 2024 au\u00a0 27 novembre 2024<\/h3>\n

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Silvina Pessino (IICAR-CONICET, Argentine) a \u00e9t\u00e9 d\u00e9tach\u00e9e \u00e0 l’UMIL, Italie, du 2 octobre au 27 novembre 2024, pour \u00e9tudier le r\u00f4le de plusieurs AUXIN RESPONSE FACTORS (ARF) et de leurs r\u00e9gulateurs miRNA respectifs dans la sp\u00e9cification de la lign\u00e9e germinale femelle chez Arabidopsis thaliana<\/em>, en collaboration avec les Dr. Lucia Colombo et Marta Mendes. En particulier, au cours de ce d\u00e9tachement, elle a particip\u00e9 aux \u00e9tapes finales de la publication de l’article Pessino SC et al.<\/em> (2024) AUXIN RESPONSIVE FACTOR 10 insensitive to miR160 regulation induces apospory-like phenotypes in Arabidopsis, iScience; <\/em><\/a>pr\u00e9par\u00e9 plusieurs livrables du projet MAD, et analys\u00e9 les donn\u00e9es McSeED et RNA-seq indiquant le contr\u00f4le de l’expression de l’ARF m\u00e9di\u00e9 par le syst\u00e8me RdDM.<\/p>\n

Photo: marta mendes (gauche) & Silvina Pessinio (droite)<\/p>\n

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Dr. Juan Pablo A. Ortiz<\/h2>\n

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IICAR, Rosario, Argentine<\/h3>\n

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Universit\u00e9 de Padoue (Italie), du 1er octobre 2024 au 1er novembre 2024<\/h3>\n

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Ce nouveau d\u00e9tachement de Juan Pablo A. Ortiz a pour objectif d’analyser des s\u00e9quences li\u00e9es \u00e0 l’apomixie, avec un accent particulier sur la d\u00e9couverte de g\u00e8nes associ\u00e9s \u00e0 la parth\u00e9nogen\u00e8se. Ce travail comprendra l’analyse de bases de donn\u00e9es transcriptomiques de g\u00e9notypes sexu\u00e9s et apomictiques afin d’identifier les g\u00e8nes qui participent aux cascades de r\u00e9actions hormonales, aux diff\u00e9rentes \u00e9tapes\u00a0 de traitement de l’ARN et \u00e0 la signalisation cellulaire. L’analyse inclura \u00e9galement les ressources g\u00e9nomiques disponibles sur les locus de contr\u00f4le de l’apomixie disponibles pour diff\u00e9rentes plantes apomictiques. Ce travail sera r\u00e9alis\u00e9 en collaboration avec le Dr Giovanni Gabelli du groupe DAFNAE. Les r\u00e9sultats obtenus contribueront aux WP3 et WP4 et pourraient fournir de nouveaux candidats pour l’induction de la parth\u00e9nogen\u00e8se chez les esp\u00e8ces apomictiques naturelles.<\/p>\n

Photo: Partage du mat\u00e9… de gauche \u00e0 droite: Juan Pablo Ortiz, Lorena Siena & Samela Draga<\/p>\n

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Dr. Lorena Siena<\/h2>\n

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IICAR, Rosario, Argentine<\/h3>\n

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IBBR (Italie), du 30 ao\u00fbt 2024 au 29 septembre 2024<\/h3>\n

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Pendant sa visite au laboratoire du Dr. Fulvio Pupilli, Lorena s\u00e9quencera des plantes t\u00e9traplo\u00efdes sexuelles et apomictiques de Paspalum simplex afin d’am\u00e9liorer les assemblages actuels de g\u00e9nomes t\u00e9traplo\u00efdes. Les informations produites contribueront au WP3 et seront utilis\u00e9es pour \u00e9tudier la structure et l’\u00e9volution du g\u00e9nome et pour d\u00e9chiffrer le contenu g\u00e9nique du locus d’apomixie. Ce travail sera r\u00e9alis\u00e9 en collaboration avec le Dr Andrea Rubini et les BC Davide Perrone de l’IBBR et le Dr Juan Pablo A Ortiz de l’IICAR. Lorena mettra \u00e9galement en place des tests d’hybridation in situ pour caract\u00e9riser le profil d’expression du g\u00e8ne candidat ARF5 de P. simplex dans les ovaires apomictiques et sexuels. Elle participera \u00e0 la conception et \u00e0 l’\u00e9tiquetage de la sonde, ainsi qu’au montage en paraffine des ovaires. Le sectionnement et l’hybridation seront effectu\u00e9s \u00e0 l’IICAR, CONICET-UNR. Ces exp\u00e9riences seront r\u00e9alis\u00e9es en collaboration avec Dr Eugenia C\u00e1ceres de l’IBBR.<\/p>\n

Image: Lorena Siena (droite) & Davide Perrone (gauche)<\/p>\n

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Dr. Juan Pablo A. Ortiz<\/h2>\n

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Rosario, Argentina, IICAR<\/h3>\n

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IBBR (Italie), du 30 ao\u00fbt 2024 au 29 septembre 2024<\/h3>\n

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L’objectif de ce d\u00e9tachement est d’approfondir la caract\u00e9risation des g\u00e9nomes sexu\u00e9s et apomictiques chez Paspalum simplex. Le plan de travail comprend : extractions d’ADN, pr\u00e9paration de biblioth\u00e8ques et s\u00e9quen\u00e7age ONT de plantes t\u00e9traplo\u00efdes. Ces activit\u00e9s contribueront au WP3, qui vise \u00e0 analyser la structure et l’\u00e9volution des g\u00e9nomes apomictiques et \u00e0 \u00e9lucider le contenu en g\u00e8nes et la fonction du locus d’apomixie. Les autres membres de l’\u00e9quipe qui participeront \u00e0 ce travail sont : Dr Lorena Siena (IICAR), Dr Andrea Rubini (IBBR) et BSc Davide Perrone (IBBR). Juan Pablo travaillera \u00e9galement \u00e0 la construction d’un vecteur RNAi con\u00e7u pour r\u00e9duire l’expression de ORC3, un g\u00e8ne candidat identifi\u00e9 par le groupe du Dr Pupilli comme r\u00e9gulateur du d\u00e9veloppement de l’albumen dans les graines apomictiques. Cette construction sera utilis\u00e9e pour g\u00e9n\u00e9rer des plantes de ma\u00efs transg\u00e9niques afin de valider sa fonction dans le contr\u00f4le de l’exc\u00e8s g\u00e9nomique maternel dans l’albumen d’une plante sexu\u00e9e cultiv\u00e9e. Cette partie du travail sera r\u00e9alis\u00e9e en collaboration avec Dra Francesca De Marchis et Dr Michelle Bellucci, de l’IBBR.\u00a0<\/p>\n

Image: Juan Pablo A. Ortiz (gauche) & Fulvio Pupilli (droite)<\/p>\n

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Ing. David Lopez Villegas<\/h2>\n

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CERZOS, Bahia Blanca, Argentine<\/h3>\n

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IRD (France), du 20 juin 2024 au 26 ao\u00fbt 2024<\/h3>\n

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David Lopez Villegas (Bahia Blanca, Argentine) a effectu\u00e9 un d\u00e9tachement \u00e0 l’IRD \u00e0 Montpellier, en France, du 24 juin au 23 ao\u00fbt 2024. Au cours de ce d\u00e9tachement, il a acquis les comp\u00e9tences et les connaissances n\u00e9cessaires pour mener \u00e0 bien la valorisation technologique en collaboration avec le Bureau de transfert de technologie de l’IRD, en mettant l’accent sur l’apomixie et ses applications potentielles dans l’agriculture. Il a proc\u00e9d\u00e9 \u00e0 une analyse syst\u00e9matique des brevets li\u00e9s \u00e0 l’apomixie et aux technologies associ\u00e9es, fournissant ainsi des informations pr\u00e9cieuses sur les tendances technologiques actuelles, les strat\u00e9gies en mati\u00e8re de propri\u00e9t\u00e9 intellectuelle et les principales institutions et entreprises actives dans ce domaine.<\/p>\n

Photo: David Lopez, IRD, Montpellier, France.<\/p>\n

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Dr. Andr\u00e9s Bellido<\/h2>\n

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CERZOS, Bahia Blanca, Argentine<\/h3>\n

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IRD (France), du 31 mai 2024 au 29 juin 2024<\/h3>\n

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Des plantes transg\u00e9niques d’Arabidopsis thaliana <\/em>ont \u00e9t\u00e9 g\u00e9n\u00e9r\u00e9es en exprimant des g\u00e8nes li\u00e9s \u00e0 l’apomixie et identifi\u00e9s chez Eragrostis curvula<\/em>. Celles-ci expriment plusieurs ph\u00e9notypes li\u00e9s \u00e0 l’apomixie lors de la sp\u00e9cification de la CMM et au cours du development du sac embryonnaire, ainsi que des signes de perte de m\u00e9thylation de l’ADN. L’objectif de ce travail est de caract\u00e9riser les marques d’histones les plus pertinentes au cours du d\u00e9veloppement de l’ovule dans ces lign\u00e9es transg\u00e9niques d’Arabidopsis<\/em> en utilisant des techniques d’immunomarquage et de microscopie confocale.\u00a0<\/p>\n

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Dr. Viviana Echenique<\/h2>\n

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CERZOS, Bahia Blanca, Argentine<\/h3>\n

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Universit\u00e9 de P\u00e9rouse (Italie), du 30 mai 2024 au 2 ao\u00fbt 2024<\/h3>\n

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L’objectif de ce d\u00e9tachement \u00e0 l’UNIPG est de poursuivre le travail avec le groupe du Dr. Albertini pour identifier la r\u00e9gion g\u00e9nomique impliqu\u00e9e dans l’apomixie chez Eragrostis curvula<\/em> et pour caract\u00e9riser les g\u00e8nes pr\u00e9sents. Pour cela, nous analyserons les donn\u00e9es pr\u00e9c\u00e9demment obtenues \u00e0 partir du pang\u00e9nome d’E. curvula<\/em>, qui consiste en un s\u00e9quen\u00e7age Illumina de 10 g\u00e9notypes pr\u00e9sentant des g\u00e9nomes similaires mais avec des diff\u00e9rences pour les modes de reproduction (apomictique et sexu\u00e9) et les niveaux de plo\u00efdie (2x, 4x, 6x, 7x et 8x). Les donn\u00e9es seront utilis\u00e9es pour \u00e9tudier les r\u00e9gions g\u00e9nomiques impliqu\u00e9es dans l’apomixie et leur synt\u00e9nie avec leurs r\u00e9gions homologues sexu\u00e9es par une approche de cartographie afin d’obtenir le premier assemblage du pang\u00e9nome d’E. curvula<\/em>.<\/p>\n

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Dr. Maria Cielo Pasten<\/h2>\n

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CERZOS, Bahia Blanca, Argentine<\/h3>\n

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Universit\u00e9 de P\u00e9rouse (Italie), du 30 mai 2024 au 2 ao\u00fbt 2024<\/h3>\n

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L’objectif de ce d\u00e9tachement \u00e9tait d’initier la caract\u00e9risation de lign\u00e9es mutantes SALK chez Arabidopsis thaliana<\/em> pour les g\u00e8nes CHC2 (Clathrin heavy chain 2) et NFD3 (Nuclear fusion defective 3), tous deux pr\u00e9c\u00e9demment identifi\u00e9s comme exprim\u00e9s de mani\u00e8re diff\u00e9rentielle dans les g\u00e9notypes apomictiques et sexu\u00e9s d’Eragrostis curvula<\/em>. Le g\u00e9notypage avec des amorces sp\u00e9cifiques a \u00e9t\u00e9 r\u00e9alis\u00e9 et plusieurs des lign\u00e9es h\u00e9t\u00e9rozygotes qui ont \u00e9t\u00e9 identifi\u00e9es sont maintenant \u00e9tudi\u00e9es. Nous avons \u00e9galement obtenu des plantes mut\u00e9es par CRISPR-Cas9 pour le g\u00e8ne SPL7 (Squamosa promoter binding like 7). Celles-ci sont en cours de caract\u00e9risation. De plus, des hybridations in situ<\/em> (ISH) ont \u00e9t\u00e9 r\u00e9alis\u00e9es afin de determiner le profil d\u2019expression du g\u00e8ne SPL7\u00a0 dans les tissus reproducteurs d\u2019individus diplo\u00efdes et t\u00e9traplo\u00efdes chez A. thaliana <\/em>(ecotype Col0).<\/p>\n

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Domenico Loperfido, MSc<\/h2>\n

 <\/p>\n

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Universit\u00e9 de Milan, Italie<\/h3>\n

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CERZOS (Bahia Blanca, Argentine), du 11 mars 2024 au 8 juin 2024<\/h3>\n

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Au cours des trois mois (du 11 mai au 8 juin 2024) qu’il a pass\u00e9s au CERZOS, Domenico Loperfido a travaill\u00e9 dans le laboratoire du professeur Echenique sous la supervision de Jos\u00e9 Carballo et de Cristian Gallo du CERZOS et celle du professeur Ezquer de l’Universit\u00e9 de Milan. L’objectif du d\u00e9tachement \u00e9tait d’\u00e9tudier les g\u00e8nes candidats impliqu\u00e9s dans la croissance et le d\u00e9veloppement des graines par des \u00e9tudes d’association visant \u00e0 comprendre le r\u00f4le que ces g\u00e8nes peuvent avoir dans l’h\u00e9r\u00e9dit\u00e9 des processus apomictiques. Les g\u00e9nomes de diff\u00e9rentes accessions d’Arabidopsis thaliana <\/em>ont \u00e9t\u00e9 utilis\u00e9s ; en utilisant des outils bioinformatiques, par exemple bcftools, vcftools, R, GAPIT, et d’autres, des SNP significatifs dans les g\u00e8nes ph\u00e9notypiquement associ\u00e9s \u00e0 la croissance et au d\u00e9veloppement des composants de la graine (embryon, endosperme, et t\u00e9gument) contr\u00f4lant la vigueur hybride dans les plantes sexu\u00e9es ont \u00e9t\u00e9 identifi\u00e9s.<\/p>\n

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Dr. Diego Zappacosta <\/h2>\n

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CERZOS, Bahia Blanca, Argentine<\/h3>\n

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Universit\u00e9 de P\u00e9rouse (Italie), du 5 mars 2024 au 5 mai 2024<\/h3>\n

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L’objectif principal de ce d\u00e9tachement est de poursuivre la caract\u00e9risation d’une population en segregation pour l\u2019apomixie chez Eragrostis curvula pour g\u00e9n\u00e9rer une carte de liaison et s\u00e9lectioner des individus pr\u00e9sentants des marqueurs mol\u00e9culaires similaires mais des modes de reproduction diff\u00e9rents (apomictique et sexu\u00e9) pour de futures analyses transcriptomiques.\u00a0Pendant ces deux mois, en collaboration avec le groupe de l’UNIPG, je vais ph\u00e9notyper et caract\u00e9riser mol\u00e9culairement des individus de la population de cartographie. Notre objectif est de cr\u00e9er une nouvelle carte consensus pour E. curvula<\/em>, d’identifier des marqueurs mol\u00e9culaires li\u00e9s \u00e0 l’apom\u00e9iose et, finalement, de d\u00e9couvrir des g\u00e8nes qui pourraient jouer un r\u00f4le dans le contr\u00f4le de la reproduction apomictique. De plus, je vais aussi contribuer \u00e0 la production d’un calendrier visant \u00e0 associer les param\u00e8tres morphologiques floraux aux stades de d\u00e9veloppement de la m\u00e9gasporogen\u00e8se et de la m\u00e9gagam\u00e9togen\u00e8se pour diff\u00e9rents g\u00e9notypes apomictiques et sexu\u00e9s chez E. curvula<\/em> (Task 3.3).<\/p>\n

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Lucia Giordano, doctorante<\/h2>\n

 <\/p>\n

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Universit\u00e9 de P\u00e9rouse, Italie<\/h3>\n

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Universit\u00e9 d’Ad\u00e9la\u00efde (Australie), du 18 janvier 2024 au 2 juin 2024<\/h3>\n

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2023<\/h2>\n

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Samela Draga, doctorante<\/h2>\n

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Universit\u00e9 de Padoue, Italie<\/h3>\n

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IICAR (Rosario, Argentine), du 3 novembre 2023 au\u00a0 19 janvier 2024<\/h3>\n

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La visite de Samela Draga au laboratoire du Prof. Pessino du 3 novembre 2023 au 18 janvier 2024 apour objectif g\u00e9n\u00e9ral de poursuivre le projet initi\u00e9 en mars-juillet 2023 \u00e0 l’IICAR-CONICET-UNR qui vise \u00e0 comprendre le r\u00f4le du facteur d’\u00e9pissage BUD13 (un interacteur de TGS1) chez les plantes de Paspalum apomictiques et sexu\u00e9es. BUD13 pr\u00e9sente une expression diff\u00e9rentielle dans les fleurs des plantes sexu\u00e9es et apomictiques chez au moins deux esp\u00e8ces aposporiques, Paspalum notatum<\/em> et Hypericum perforatum<\/em>. Il est int\u00e9ressant de noter que les mutants bud13 d’Arabidopsis sont associ\u00e9s \u00e0 des d\u00e9fauts du d\u00e9veloppement embryonnaire pr\u00e9coce. Au cours de sa visite, Samela produira une sonde BUD13 marqu\u00e9e \u00e0 la digoxig\u00e9nine, pr\u00e9parera des coupes d\u2019ovaires inclus dans des blocs de paraffine, et effectuera l’analyse ISH. Les observations permettront de d\u00e9terminer les profils d’expression spatiale de BUD13 dans les organes reproducteurs des g\u00e9notypes sexuels et apomictiques de Paspalum notatum<\/em>.<\/p>\n

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Dr. Giovanni Gabelli<\/h2>\n

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Universit\u00e9 de P\u00e9rouse, Italie<\/h3>\n

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IICAR (Rosario, Argentine), du 15 novembre 2023 au 15 d\u00e9cembre 2023<\/h3>\n

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Dr. Fulvio Pupilli<\/h2>\n

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IBBR, P\u00e9rouse, Italie<\/h3>\n

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IICAR (Rosario, Argentine), du 8 novembre 2023 au\u00a0 9 d\u00e9cembre 2024<\/h3>\n

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Au cours de son premier d\u00e9tachement \u00e0 Rosario du 9 novembre au 14 d\u00e9cembre 2023, Fulvio Pupilli a discut\u00e9 avec Juan Pablo Ortiz, son \u00e9quipe et Olivier Leblanc les r\u00e9centes avanc\u00e9es concernant le s\u00e9quen\u00e7age Nanopore et l’assemblage de g\u00e9nomes apomictique et sexu\u00e9 chez Paspalum simplex<\/em>. De plus, les progr\u00e8s r\u00e9alis\u00e9s dans la caract\u00e9risation du g\u00e8ne PsARF5 qui est exprim\u00e9 de mani\u00e8re diff\u00e9rentielle entre les tissus du nucelle diss\u00e9qu\u00e9 par LCM de g\u00e9notypes apomictiques et sexuels ont \u00e9t\u00e9 discut\u00e9s avec JP Ortiz ainsi que les interactions possibles avec PnIAA30, un g\u00e8ne en cours de caract\u00e9risation fonctionnelle \u00a0\u00e0 l’IICAR. Une s\u00e9rie d’exp\u00e9riences d’hybridation in situ<\/em> pour PsARF5 a \u00e9t\u00e9 programm\u00e9e pour le prochain d\u00e9tachement. Enfin, il a pr\u00e9par\u00e9 un expos\u00e9 sur le r\u00f4le du g\u00e8ne PsORC3 dans le d\u00e9veloppement des l\u2019albumens pr\u00e9sentant un exc\u00e8s g\u00e9nomique maternel chez P. simplex<\/em> et un hommage au professeur Camilo Luis Quarin pour le IVe congr\u00e8s Apomixis, qui se tiendra du 3 au 7 d\u00e9cembre 2023 \u00e0 Rosario, en Argentine.<\/p>\n

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Giacomo Bongiorno, doctorant<\/h2>\n

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Universit\u00e9 d P\u00e9rouse, Italie<\/h3>\n

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CERZOS (Bahia Blanca, Argentine), du 18 mai 2023 au 9 d\u00e9cembre 2023<\/h3>\n

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Pendant ce d\u00e9tachement au CERZOS, je me suis principalement concentr\u00e9 sur la gramin\u00e9e diplosporique Eragrostis curvula<\/em> dans le but de construire un pan-g\u00e9nome complet. L’utilisation du cultivar Victoria comme g\u00e9nome de r\u00e9f\u00e9rence a fourni une base solide pour \u00e9tudier la variation g\u00e9nomique, les relations phylog\u00e9n\u00e9tiques et l’impact de la plo\u00efdie sur l’\u00e9volution du g\u00e9nome et les modes de reproduction. Cette \u00e9tude pan-g\u00e9nomique contribue de mani\u00e8re significative \u00e0 notre compr\u00e9hension de la diversit\u00e9 g\u00e9nomique d’E. curvula<\/em>, comme ressource d\u2019int\u00e9r\u00eat pour des investigations cibl\u00e9es sur les m\u00e9canismes mol\u00e9culaires r\u00e9gissant ses modes de reproduction et ses relations avec la plo\u00efdie.<\/p>\n

Photo (de gauche \u00e0 droite): Juan Pablo Selva, Diego Zappacosta, Juan Manuel Rodrigo, Nengi Lawson, Viviana Echenique, Alejandra Diaz & Giacomo Bongiorno<\/p>\n

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Dr. Daphn\u00e9 Autran<\/h2>\n

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IRD, Montpellier, France<\/h3>\n

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IICAR (Rosario, Argentine), du 22 octobre 2023 au 17 d\u00e9cembre 2023<\/h3>\n

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Au cours de ce d\u00e9tachement, nous poursuivons le travail sur Paspalum rufum avec Luciana Delgado.\u00a0 Pour comprendre les interrelations entre l’architecture des primordia de l’ovule et la plasticit\u00e9 du destin cellulaire menant \u00e0 l’aposporie, nous effectuons une analyse morphom\u00e9trique cellulaire en 3D des g\u00e9notypes de P. rufum montrant une expressivit\u00e9 diff\u00e9rentielle de l’aposporie. La premi\u00e8re \u00e9tape consiste \u00e0 construire un atlas 3D de r\u00e9f\u00e9rence du g\u00e9notype sexu\u00e9. Apr\u00e8s le travail de segmentation, d’\u00e9tiquetage et d’exportation des donn\u00e9es effectu\u00e9 lors des pr\u00e9c\u00e9dents d\u00e9tachements \u00e0 l’aide du logiciel MorphographX (MGX), nous avons mis en place une analyse quantitative sur un groupe pilote de 11 ovules \u00e0 des stades successifs couvrant la fen\u00eatre de d\u00e9veloppement qui nous int\u00e9resse. En plus de la quantification du nombre et de la taille des cellules, diff\u00e9rents descripteurs de forme, au niveau des cellules et des organes, ont \u00e9t\u00e9 s\u00e9lectionn\u00e9s et calcul\u00e9s \u00e0 partir des caract\u00e9ristiques extraites par MGX. Comme les donn\u00e9es pilotes comprennent d\u00e9j\u00e0 des milliers de cellules \u00e0 analyser, des scripts personnalis\u00e9s en R ont \u00e9t\u00e9 \u00e9crits pour compiler, visualiser et analyser statistiquement les donn\u00e9es. En outre, les ovules de P. rufum au stade de la m\u00e9iose ont \u00e9t\u00e9 diss\u00e9qu\u00e9s, fix\u00e9s et nettoy\u00e9s, afin d’\u00e9tendre la fen\u00eatre de d\u00e9veloppement couverte et de maximiser la probabilit\u00e9 de d\u00e9tection de la croissance cellulaire diff\u00e9rentielle entre les g\u00e9notypes sexu\u00e9s et aposporiques.<\/p>\n

Paspalum rufum<\/em>, Luciana Delgado & Daphn\u00e9 Autran<\/p>\n

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Dr. Olivier Leblanc<\/h2>\n

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IRD, Montpellier, France<\/h3>\n

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IICAR (Rosario, Argentine), du 15 octobre 2023 au 13 d\u00e9cembre 2023<\/h3>\n

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Cette deuxi\u00e8me visite \u00e0 l’IICAR s\u2019inscrit dans un d\u00e9tachement consacr\u00e9 \u00e0 la g\u00e9nomique de l’apomixie (WP3) et \u00e0 la caract\u00e9risation fonctionnelle de g\u00e8nes candidats de Paspalum<\/em> pour l’apomixie en utilisant des mutants d’Arabidopsis <\/em>(WP4). En ce qui concerne les approches g\u00e9nomiques, une premier objectif est de finaliser la r\u00e9daction d\u2019un premier article rapportant l’assemblage et l’annotation d\u00e9taill\u00e9e d’un g\u00e9nome diplo\u00efde de r\u00e9f\u00e9rence de P. notatum<\/em>. Par ailleurs, nous concentrerons nos analyses sur la compr\u00e9hension des variations entre le g\u00e9nome des plantes diplo\u00efdes sexu\u00e9es et celui des plantes t\u00e9traplo\u00efdes apomictiques en utilisant des approches de g\u00e9nomique comparative et de k-mers. En ce qui concerne la caract\u00e9risation fonctionnelle des mutants d’Arabidopsis<\/em>, les effets des mutations tgs1<\/em> sur l’\u00e9pissage de l’ARN seront analys\u00e9es \u00e0 l’aide d’analyses transcriptionnelles comparatives et d’outils cytologiques (rapporteurs fluorescents en cours de construction). Par ailleurs, je participe \u00e0 l’organisation du deuxi\u00e8me atelier MAD sur le s\u00e9quen\u00e7age et la bioinformatique de l’ONT (Rosario, 20-25 novembre) et de la conf\u00e9rence Apomixis2023 (Rosario, 3-7 d\u00e9cembre).<\/p>\n

Photo: visite de vieilles connaissances \u00e0 Cayasta (Santa Fe, Argentine), berceau de la famille Paspalum. De gauche \u00e0 droite : \u00a0O Leblanc, JPA Ortiz, & L Siena<\/p>\n

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Davide Perrone<\/h2>\n

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Universit\u00e9 de Milan, Italie<\/h3>\n

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IICAR (Rosario, Argentine), du 12 septembre 2023 au 13 d\u00e9cembre 2023<\/h3>\n

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Davide Perrone est en visite \u00e0 l’IICAR-CONICET pour poursuivre les exp\u00e9riences initi\u00e9es lors d’un pr\u00e9c\u00e9dent d\u00e9tachement, concernant la transformation de plantes apomictiques et sexu\u00e9es de Paspalum notatum<\/em> avec des rapporteurs fluorescents d’auxine et de cytokinine. Le but de ces exp\u00e9riences est de comparer la distribution des hormones dans les ovules de plantes sexu\u00e9es et apomictiques. De plus, Davide initiera des exp\u00e9riences d’hybridation in situ<\/em> visant \u00e0 comparer l’expression du gene AUXIN RESPONSE FACTOR 10 (ARF10) dans les ovules des biotypes sexu\u00e9s et apomictiques de Paspalum notatum<\/em>. Enfin, en collaboration avec les scientifiques de l’IICAR, il conduira des analyses miR-seq en utilisant les donn\u00e9es g\u00e9n\u00e9r\u00e9es pr\u00e9c\u00e9demment \u00e0 Milan.<\/p>\n

Photo (de gauche \u00e0 droite): Augustus Villalba (IBONE), Silvina Pessino (IICAR), Maricel Podio (IICAR) & Davidse Perrone (Univ. Milan)<\/p>\n

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Dr. Cristian Gallo<\/h2>\n

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Bahia Blanca, Argentina, CERZOS<\/h3>\n

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Universit\u00e9 de P\u00e9rouse (Italie), du 1er septembre 2023 au 30 octobre 2023<\/h3>\n

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Dr. Francesca De Marchis<\/h2>\n

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P\u00e9rouse, Italie, CNR-IBBR<\/h3>\n

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IICAR (Rosario, Argentine), du 11 ao\u00fbt 2023 au 12 septembre 2023<\/h3>\n

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Francesca De Marchis est une chercheuse du Conseil national de la recherche, CNR- IBBR \u2013 Perugia. Pendant son s\u00e9jour, elle \u00a0va collaborer avec Juan Pablo Ortiz (IICAR) et\u00a0 son expertise en biotechnologie v\u00e9g\u00e9tale sera mise \u00e0 profit pour concevoir un vecteur de transformation pour \u00a0Paspalum notatum. Certains g\u00e8nes candidats pour le d\u00e9clenchement de la parth\u00e9nogen\u00e8se, et identifi\u00e9s par le groupe de recherche de l’IICAR, seront s\u00e9lectionn\u00e9s pour la transformation de plantes par la technologie biolistique dans le but de modifier leur profil d\u2019expression (expression ectopique ou repression).<\/p>\n

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Dr. Alejendra Diaz<\/h2>\n

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Bahia Blanca, Argentine, CERZOS<\/h3>\n

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NIAB (Royaume Uni), du 1er aout 2023 au 2 octobre 2023<\/h3>\n

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Notre groupe de recherche CERZOS a engag\u00e9 une collaboration depuis plusieurs ann\u00e9es avec le groupe du Dr. Caccamo au NIAB visant \u00e0 l’identification et la caract\u00e9risation d’une r\u00e9gion g\u00e9nomique impliqu\u00e9e dans l’apomixie chez Eragrostis curvula<\/em> (Schrad.) Nees, et de ses g\u00e8nes. Certains d’entre eux, identifi\u00e9s pr\u00e9c\u00e9demment par notre groupe de recherche comme impliqu\u00e9s dans l\u2019apomixie et pour lesquels aucunne annotation chez d’autres esp\u00e8ces n\u2019est disponible, sont en cours de caract\u00e9risation fonctionnelle. L’expression de ces candidats sous des promoteurs constitutifs dans la plante mod\u00e8le qu’est le riz est l’une des approches utilis\u00e9es pour leur caract\u00e9risation. L’objectif de ce d\u00e9tachement au NIAB \u00e9tait de faire progresser la caract\u00e9risation de l’un de nos g\u00e8nes candidats, principalement pour d\u00e9terminer si sa surexpression entra\u00eenait un d\u00e9faut dans le d\u00e9veloppement du sac embryonnaire. Pour ce faire, j’ai particip\u00e9 au criblage de tissus reproducteurs provenant d’\u00e9pillets de lign\u00e9es de riz transg\u00e9niques ind\u00e9pendantes g\u00e9n\u00e9r\u00e9es au NIAB et surexprimant le g\u00e8ne d’int\u00e9r\u00eat (WP4). Nous avons utilis\u00e9 la microscopie \u00e0 contraste d’interf\u00e9rence diff\u00e9rentiel (DIC) pour observer les tissus reproducteurs nettoy\u00e9s avec une solution de salicylate de m\u00e9thyle, car cette technique permet des observations \u00e0 grande \u00e9chelle sur la m\u00e9iose et le d\u00e9veloppement du sac embryonnaire dans des ovules intacts. Ainsi, la collaboration entre le NIAB et le CERZOS nous permettra de caract\u00e9riser plus rapidement les g\u00e8nes candidats \u00e0 l’apomixie identifi\u00e9s chez Eragrostis<\/em>.<\/p>\n

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Dr. Viviana Echenique<\/h2>\n

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Bahia Blanca, Argentine, CERZOS<\/h3>\n

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NIAB (Royaume Uni), du 1er ao\u00fbt 2023 au 2 octobre 2023<\/h3>\n

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L’objectif de mon d\u00e9tachement au NIAB est de poursuivre le travail initi\u00e9 avec le groupe du Dr. Caccamo visant l’identification et la caract\u00e9risation d’une r\u00e9gion g\u00e9nomique et de g\u00e8nes impliqu\u00e9s dans l’apomixie. Diff\u00e9rentes strat\u00e9gies et de nouvelles experiences ont \u00e9t\u00e9 discut\u00e9es. Le travail en cours consiste \u00e0 s\u00e9quencer et \u00e0 assembler le g\u00e9nome du cultivar apomictique Don Walter (Eragrostis curvula<\/em>). Ce g\u00e9nome a \u00e9t\u00e9 s\u00e9quenc\u00e9 au NIAB \u00e0 l’aide de la plateforme Oxford nanopore avec le protocole de mod\u00e8le v\u00e9g\u00e9tal de tr\u00e8s haute pr\u00e9cision et l’un des \u00e9tudiants du CERZOS travaille ici \u00e0 son assemblage. Nous sommes \u00e9galement impliqu\u00e9s dans un projet de transcriptomique utilisant des donn\u00e9es RNA-seq obtenues \u00e0 partir de tissus de pistil de plantes apomictiques et sexu\u00e9es \u00e0 trois stades de d\u00e9veloppement diff\u00e9rents. Nous recherchons des g\u00e8nes diff\u00e9rentiellement exprim\u00e9s entre des plantes ayant des modes de reproduction diff\u00e9rents et des stades de d\u00e9veloppement diff\u00e9rents.<\/p>\n

Photo: Viviana devant The Eagle \u00e0 Cambridge (Angleterre)<\/p>\n

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Sol Vega, doctorante<\/h2>\n

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Rosario, Argentina, IICAR<\/h3>\n

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IRD (France), du 20 juillet 2023 au 20 septembre 2023<\/h3>\n

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Ce d\u00e9tachement de Mar\u00eda Sol \u00e0 l’IRD \u00e0 pour objectif de poursuivre la caract\u00e9risation fonctionnelle du g\u00e8ne IAA16 chez Arabidopsis thaliana, dont l’orthologue (IAA30) a \u00e9t\u00e9 associ\u00e9 \u00e0 l’apomixie chez Paspalum notatum. Les activit\u00e9s seront men\u00e9es dans le laboratoire du Dr. Olivier Leblanc et viseront l\u2019obtention de plantes double-t homozygotes issues de croisements entre le mutant iaa16<\/em> et une lign\u00e9e portant le rapporteur gam\u00e9tophytique pWOX2-CENH3-GFP<\/em> ; des plantes mutantes tgs1<\/em> (li\u00e9es \u00e0 des alt\u00e9rations du d\u00e9veloppement des gam\u00e9tophytes), et des plantes mutantes topless<\/em> (r\u00e9ponse \u00e0 l\u2019auxine au cours de l’embryogen\u00e8se). Des analyses ph\u00e9notypiques du d\u00e9veloppement reproducteur des mat\u00e9riels obtenus seront effectu\u00e9es en utilisant la microscopie \u00e0 fluorescence ou DIC pendant le d\u00e9tachement et ensuite \u00e0 l’IICAR. Par ailleurs, l’\u00e9tude de mutants pour d’autres g\u00e8nes du clade IAA16 (iaa14<\/em> et iaa17<\/em>) ainsi que pour IAA16 a \u00e9t\u00e9 initi\u00e9e.<\/p>\n

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Dr. Lorena Siena<\/h2>\n

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Rosario, Argentina, IICAR<\/h3>\n

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IRD (France), du 22 juin 2023 au 16 septembre 2023<\/h3>\n

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Dans le cadre de ce d\u00e9tachement, Lorena Siena, de l’IICAR, sera accueillie \u00e0 l’IRD pendant trois mois. L’objectif principal est de faire progresser la caract\u00e9risation fonctionnelle des g\u00e8nes TGS1 et QGJ au cours du d\u00e9veloppement reproducteur chez Arabidopsis thaliana<\/em>. Les activit\u00e9s seront men\u00e9es en collaboration avec le groupe de l’IRD e viseront, tout d’abord, \u00e0 explorer le potentiel pour la formation d\u2019embryons parth\u00e9nog\u00e9n\u00e9tiques dans les lign\u00e9es mutantes tgs1<\/em> en utilisant des rapporteurs fluorescents sp\u00e9cifiques de l’embryon et, transmis maternellement ou paternellement. En outre, l’identit\u00e9 cellulaire des m\u00e9gaspores, suppl\u00e9mentaires et fonctionnelles, observ\u00e9es dans les ovules qgj<\/em> sera caract\u00e9ris\u00e9e de mani\u00e8re approfondie, par exemple en utilisant le rapporteur pWOX2-CENH3-GFP<\/em>. De plus, nous initierons des exp\u00e9riences visant \u00e0 identifier les interactions potentielles entre QGJ et TGS1 en d\u00e9terminant le profil d’expression de QGJ (pQGJ-GUS) dans les ovules tgs1<\/em> et, r\u00e9ciproquement, celui de TGS1 (pTGS1:TGS1-GUS<\/em>) dans les ovules qgj<\/em>.<\/p>\n

Image: Aqueduc de Montpellier (@ Lorena Siena, IICAR)<\/p>\n

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Dr. Jose Carballo<\/h2>\n

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Bahia Blanca, Argentina, CERZOS<\/h3>\n

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NIAB (Royaume Uni), du 14 juin 2023 au 14 septembre 2023<\/h3>\n

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L’objectif de ce d\u00e9tachement au NIAB est de poursuivre le s\u00e9quen\u00e7age et l’assemblage du g\u00e9nome apomictique d’Eragrostis curvula<\/em>. Le g\u00e9nome du g\u00e9notype Don Walter a \u00e9t\u00e9 s\u00e9quenc\u00e9 au NIAB en utilisant la plateforme Oxford nanopore avec le protocole du mod\u00e8le de plante de tr\u00e8s haute pr\u00e9cision. Les lectures ont \u00e9t\u00e9 assembl\u00e9es \u00e0 l’aide du logiciel Flye et \u00e9chafaud\u00e9es avec la technologie Omni-C afin d’obtenir un assemblage \u00e0 l’\u00e9chelle du chromosome. Le g\u00e9nome assembl\u00e9 a ensuite \u00e9t\u00e9 annot\u00e9 pour obtenir les sous-g\u00e9nomes A et B de l’allotetraplo\u00efde Don Walter. De plus, pendant cette visite \u00e0 Cambridge, les donn\u00e9es RNA-seq, obtenues \u00e0 partir de pistils de plantes apomictiques et sexu\u00e9es \u00e0 trois stades de d\u00e9veloppement diff\u00e9rents, ont \u00e9t\u00e9 analys\u00e9es pour identifier les g\u00e8nes diff\u00e9rentiellement exprim\u00e9s. Ce d\u00e9tachement poursuit les efforts de collaboration entre le NIAB et le CERZOS-CONICET afin de caract\u00e9riser les bases mol\u00e9culaires de l’apomixie par une approche bioinformatique.<\/p>\n

\u200b<\/p>\n

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Dr. Tamunonengiye-ofori Lawson<\/h2>\n

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Cambridge, UK, NIAB<\/h3>\n

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CERZOS (Argentine), du 20 mai 2023 au 19 juillet 2023<\/h3>\n

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L’objectif de ce s\u00e9jour au CERZOS \u00e9tait d’approfondir mes connaissances, mes comp\u00e9tences et mon exp\u00e9rience pratique dans le domaine de l’hybridation in-situ<\/em> et de la microscopie DIC chez les plantes.<\/p>\n

\u200b<\/p>\n

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Dr. Luciana Delgado<\/h2>\n

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Rosario, Argentine, IICAR<\/h3>\n

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IRD (France), du 20 mai 2023 au 19 ao\u00fbt 2023<\/h3>\n

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Cette mission s\u2019inscrit dans la continuit\u00e9 des travaux initi\u00e9s en 2022 avec Daphn\u00e9 Autran visant \u00e0 l\u2019obtention d\u2019un atlas 3D du d\u00e9veloppement pr\u00e9coce de l’ovule chez Paspalum rufum<\/em>. Nous r\u00e9aliserons la segmentation et la classification des cellules des primordia d\u2019ovules \u00e0 l’aide d’images haute r\u00e9solution obtenues pr\u00e9c\u00e9demment et un workflow partiellement automatis\u00e9 pr\u00e9alablement \u00e9tabli utilisant le logiciel libre MorphoGraphX. De plus, nous testerons diff\u00e9rents protocoles d’incorporation et d\u00e9tection d’un analogue de la thymidine (EdU) pour quantifier les divisions cellulaires au cours du d\u00e9veloppement de l’ovule chez diff\u00e9rentes gramin\u00e9es, dont deux esp\u00e8ces mod\u00e8les sexu\u00e9es (le riz et le ma\u00efs) et des plantes apomictiques de Paspalum<\/em>, \u00a0Ces protocoles doivent \u00eatre optimis\u00e9s pour les ovules de gramin\u00e9es de morphologies diverses. Les informations quantitatives ainsi collect\u00e9es \u00e0 partir de l’atlas 3D et des divisions cellulaires pour des ovules de plantes sexu\u00e9es et apomictiques am\u00e9liorera notre compr\u00e9hension de la transition entre sexualit\u00e9 et apomixie.<\/p>\n

Photo\u00a0: Cellule en interphase dans un jeune ovule de Paspalum rufum<\/em> pr\u00e9sentant un signal fluorescent de l\u2019EdU incorpor\u00e9 \u00e0 l\u2019ADN (@ Luciana Delgado, IICAR).<\/p>\n

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Samela Draga, doctorante<\/h2>\n

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Universit\u00e9 de Padoue, Italie<\/h3>\n

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IICAR (Rosario, Argentine), du 16 mai 2023 au 8 ao\u00fbt 2023<\/h3>\n

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Le travail de Samela \u00e0 l\u2019IICAR a pour objectif principal de contribuer \u00e0 l’\u00e9lucidation du r\u00f4le dans la transition reproduction sexu\u00e9e-apomixie de la prot\u00e9ine BUD13, requise pour l’\u00e9pissage alternatif. En particulier, elle caract\u00e9risera BUD13 dans les bases de donn\u00e9es transcriptomiques et g\u00e9nomiques de Paspalum notatum<\/em> et g\u00e9n\u00e9rera des sondes pour leur d\u00e9tection par hybridation in-situ des ARNs. De plus, elle classera les ovules de plantes de Paspalum notatum sexu\u00e9es et apomictiques en fonction des stades de d\u00e9veloppement en utilisant la cytoembryologie DIC, les d\u00e9shydratera dans une s\u00e9rie \u00e9thanol\/xylol et les inclura dans de la paraffine. Lors d’une deuxi\u00e8me visite (octobre-d\u00e9cembre), elle pr\u00e9parera des sections d’ovule pour hybridation avec des sondes d’ARN sens et antisens marqu\u00e9es \u00e0 la digoxig\u00e9nine et d\u00e9tect\u00e9es par r\u00e9action colorim\u00e9trique (anticorps DIG conjugu\u00e9 \u00e0 la phosphase alcaline, substrats NBT\/BCIP).\u00a0 Nous esp\u00e9rons obtenir des informations sur l’expression spatiale de ces g\u00e8nes candidats pour des g\u00e9notypes apomictiques et sexu\u00e9s.<\/p>\n

Samela Draga (droite) & Silivina Pessino (gauche)<\/p>\n

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Nicola Babolin, doctorant<\/h2>\n

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Universit\u00e9 de Milan, Italie<\/h3>\n

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Universit\u00e9 d’Ad\u00e9laide (Australie), du 10 avril 2023 au 13 octobre 2023<\/h3>\n

\u200b<\/p>\n

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Mon d\u00e9tachement \u00e0 l’Universit\u00e9 d’Ad\u00e9la\u00efde (Australie) dans le laboratoire du Prof. Matthew Tucker a pour objectif de mieux caract\u00e9riser le r\u00e9seau mol\u00e9culaire \u00e9tabli entre les deux g\u00e8nes MADS-box, STK et ABS, au cours de la f\u00e9condation et du d\u00e9veloppement de la graine chez Arabidopsis thaliana.<\/p>\n

Je serai aussi impliqu\u00e9 dans les projets men\u00e9s par l’\u00e9quipe du prof. Tucker sur l’orge concernant le d\u00e9veloppement de l’ovule et de la graine, fortement interconnect\u00e9s avec mon projet sur Arabidopsis.<\/p>\n

De gauche \u00e0 droite: Nicola Babolin, Matthew Tucker, Rosanna Petrella<\/p>\n

\u200b<\/p>\n

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Dr. Rosanna Petrella<\/h3>\n

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Universit\u00e9 de Milan, Italie<\/h3>\n

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Universit\u00e9 d’Ad\u00e9laide (Australie), du 10 avril 2023 au 13 octobre 2023<\/h3>\n

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Pendant mon d\u00e9tachement \u00e0 l’Universit\u00e9 d’Ad\u00e9la\u00efde (laboratoire de Matthew Tucker), mon travail se concentrera sur l’\u00e9lucidation des m\u00e9canismes mol\u00e9culaires d\u00e9terminant la progression de la lign\u00e9e germinale femelle chez Arabidopsis thaliana. En particulier, j’essaierai de caract\u00e9riser les principaux acteurs impliqu\u00e9s dans la d\u00e9g\u00e9n\u00e9rescence des spores et dans la s\u00e9lection et la diff\u00e9renciation fonctionnelles des m\u00e9gaspores \u00e0 la fin de la m\u00e9gasporogen\u00e8se dans l’ovule. Je d\u00e9terminerai la contribution de l’interaction entre les phytohormones en essayant de sauver les mutants d\u00e9fectueux dans ces processus cl\u00e9s en utilisant des r\u00e9gions r\u00e9gulatrices sp\u00e9cifiques actives dans diff\u00e9rents domaines de l’ovule pour piloter sp\u00e9cifiquement l’expression des g\u00e8nes candidats.<\/p>\n

De gauche \u00e0 droite: Rosanna Petrella, Skippy le Kangourou, Nicola Babolin<\/p>\n

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Mattia Spano, doctorant<\/h2>\n

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Universit\u00e9 de Milan, Italie<\/h3>\n

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LANGEBIO (Mexique) du 1er avril 2023 au 20 juillet 2023<\/h3>\n

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L’objectif de ce d\u00e9tachement est d’\u00e9tudier les m\u00e9canismes mol\u00e9culaires qui d\u00e9terminent la lign\u00e9e germinale femelle. En particulier, j’\u00e9tudierai les contributions des phytohormones, l’auxine et les cytokinines, dans la diff\u00e9renciation de la CMM. Les esp\u00e8ces \u00e9tudi\u00e9es sont Arabidopsis thaliana<\/em> et Oryza sativa<\/em> var. Kitaake<\/em>. Dans ce cadre, j’effectuerai des tests d’immunolocalisation dans diff\u00e9rents contextes pour localiser les phytohormones pendant le d\u00e9veloppement de l’ovule en utilisant le mutant spl<\/em>\/nzz<\/em> et le type sauvage d’Arabidopsis et le type sauvage d’Oryza sativa<\/em>.<\/p>\n

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Dr. Juan Pablo A. Ortiz<\/h2>\n

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Rosario, Argentine, IICAR<\/h3>\n

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IRD (France), du 7 mars 2023 au 3 avril 2023<\/h3>\n

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Un nouveau s\u00e9jour \u00e0 l’IRD du Dr. Juan Pablo A. Ortiz de l’IICAR, effectu\u00e9 dans le cadre d’un d\u00e9tachement MAD pour la caract\u00e9risation des g\u00e9nomes apomictiques (WP3), a d\u00e9but\u00e9 le 7 mars 2023. Le plan de travail comprend l’extraction d’ADN de plusieurs \u00e9chantillons de Paspalum spp, destin\u00e9 au s\u00e9quen\u00e7age Illumina pour r\u00e9aliser des analyses structurelles du g\u00e9nome. La visite est \u00e9galement consacr\u00e9e \u00e0 la pr\u00e9paration d’un nouveau manuscrit d\u00e9crivant le s\u00e9quen\u00e7age, l’assemblage avec une r\u00e9solution au niveau chromosomique et l’annotation d’un g\u00e9nome diplo\u00efde de Paspalum notatum<\/em>. Ce r\u00e9sultat, issu \u00a0d’un effort de collaboration entre diff\u00e9rents partenaires argentins, italiens et fran\u00e7ais du projet MAD, constitue une \u00e9tape cl\u00e9 pour la progression du WP3 car il servira de r\u00e9f\u00e9rence pour caract\u00e9riser les g\u00e9nomes t\u00e9traplo\u00efdes et d\u00e9terminer la structure et l’\u00e9volution de la r\u00e9gion impliqu\u00e9e dans l’apomixie. Enfin, le programme de l’atelier international sur le s\u00e9quen\u00e7age Nanopore et la bioinformatique, organis\u00e9 par le projet MAD \u00e0 Rosario, en Argentine, en novembre prochain, sera discut\u00e9 et d\u00e9cid\u00e9 au cours de cette visite.<\/p>\n

Serres v\u00e9g\u00e9tales de l’IRD. Juan Pablo A. Ortiz (droite) & Olivier Leblanc (gauche)<\/p>\n

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Dr. Alejandra D\u00edaz<\/h2>\n

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Bahia Blanca, Argentine, CERZOS<\/h3>\n

\u200b<\/p>\n

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Universit\u00e9 de Milan (Italie), du 21 janvier 2023 au 23 mars 2023<\/h3>\n

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Ce d\u00e9tachement avait pour objectif d’analyser l’expression de deux g\u00e8nes candidats impliqu\u00e9s dans le mode de reproduction apomictique dans les tissus reproducteurs chez la gramin\u00e9e Eragrostis curvula<\/em> (WP4). Pour cela, nous avons utilis\u00e9 la technique d’hybridation in situ<\/em> de l’ARN (RNA-ISH) pour d\u00e9terminer leurs profils d’expression dans les cellules d\u2019organes reproducteurs d’E. curvula<\/em> pour un g\u00e9notype apomictique et un g\u00e9notype sexu\u00e9. En outre, nous avons \u00e9galement caract\u00e9ris\u00e9 un g\u00e8ne qui montre une expression diff\u00e9rentielle entre les plantes sexu\u00e9es et apomictiques chez E. curvula<\/em> et qui est \u00e9galement exprim\u00e9 chez la plante mod\u00e8le A. thaliana<\/em>. Pour ce dernier objectif, nous avons aussi utilis\u00e9 l’ISH pour tester l’abondance et la localisation de l’ARN de ce g\u00e8ne orthologue dans les tissus reproducteurs d’E. curvula <\/em>(g\u00e9notypes apomictique et sexu\u00e9) et d’A. thaliana<\/em> (g\u00e9notypes diplo\u00efde et t\u00e9traplo\u00efde).<\/p>\n

De gauche \u00e0 droite: Marta Mendes, Lucia Colombo, Alejandra D\u00edaz and Cielo Pasten<\/p>\n

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2022<\/h2>\n

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Gregorio Orozco<\/h2>\n

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Universit\u00e9 de Milan, Italie<\/h3>\n

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Universit\u00e9 de P\u00e9rouse (Italie), du 2 novenbre 2022 au 2 d\u00e9cembre 2022<\/h3>\n

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\u200b<\/p>\n

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Dr. Lucia Colombo<\/h2>\n

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Universit\u00e9 de Milan, Italie<\/h3>\n

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IICAR (Rosario, Argentine), du 14 novembre 2022 au 27 novembre 2022<\/h3>\n

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Jimena Gallardo<\/h2>\n

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Bahia Blanca, Argentine, CERZOS<\/h3>\n

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Universit\u00e9 de P\u00e9rouse (Italie), du 2 novenbre 2022 au 2 d\u00e9cembre 2022<\/h3>\n

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Dr. Olivier Leblanc<\/h2>\n

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Montpellier, France, IRD<\/h3>\n

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IICAR (Argentine), du 17 octobre 2022 au 16 d\u00e9cembre 2022<\/h3>\n

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Ce d\u00e9tachement vise \u00e0 poursuivre (1) la caract\u00e9risation de la r\u00e9gion chromosomique associ\u00e9e \u00e0 l’apomixie chez les plantes fourrag\u00e8res tropicales. Comme nous avons maintenant termin\u00e9 l’assemblage et l’annotation du g\u00e9nome d’un individu diplo\u00efde sexu\u00e9, diff\u00e9rentes approches seront explor\u00e9es pour r\u00e9v\u00e9ler les variations g\u00e9nomiques structurelles qui peuvent sous-tendre le d\u00e9veloppement apomictique chez les esp\u00e8ces de Paspalum<\/em>. Pour cel\u00e0, nous avons produit > 100 Gb de lectures longues (technologie Nanopore) \u00e0 partir de plusieurs plantes de Paspalum apomictiques qui seront utilis\u00e9es pour l’assemblage de novo<\/em> et des analyses comparatives, et ; (2) l’analyse fonctionnelle des mutants tgs1<\/em> d’Arabidopsis<\/em> et, plus particuli\u00e8rement, l’analyse transcriptionnelle comparative dans les embryons d’Arabidopsis<\/em> tgs1 et WT collect\u00e9s \u00e0 des stades pr\u00e9coces du d\u00e9veloppement. Ces activit\u00e9s font partie du WP3 et du WP4, respectivement.<\/p>\n

Image: Clones m\u00e9talliques dans la pampa proche de Rosario, Province de Santa Fe, Argentine (@ O Leblanc)<\/p>\n

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Dr. Maria Cielo Pasten<\/h2>\n

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Bahia Blanca, Argentine, CERZOS<\/h3>\n

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Universit\u00e9 de Milan (Italie), du 30 septembre 2022 au 1er mars 2023<\/h3>\n

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L’objectif de ce d\u00e9tachement \u00e9tait d’analyser les g\u00e8nes qui montrent une expression diff\u00e9rentielle entre ovules de plantes sexu\u00e9es et apomictiques chez l’esp\u00e8ce Eragrostis curvula et, qui sont \u00e9galement pr\u00e9sents dans la plante mod\u00e8le Arabidopsis thaliana. Dans ce but, nous avons \u00e9tudi\u00e9 le ph\u00e9notype de mutants d’A. thaliana, disponibles publiquement pour certains de ces g\u00e8nes, mais aussi celui de nouvelles lign\u00e9es de mutants transg\u00e9niques g\u00e9n\u00e9r\u00e9es par la strat\u00e9gie CRISPR-Cas9. De plus, le d\u00e9veloppement des ovules dans les g\u00e9notypes apomictiques et sexuels d’E. curvula a \u00e9t\u00e9 caract\u00e9ris\u00e9 \u00e0 l’aide de la microscopie confocale afin d’approfondir nos connaissances sur les structures et les diff\u00e9rences entre les modes de reproduction sexuel et asexu\u00e9.<\/p>\n

De gauche \u00e0 droite: Marta Mendes, Lucia Colombo, Alejandra D\u00edaz and Cielo Pasten<\/p>\n

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Pr. Pablo Roncallo<\/h2>\n

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Bahia Blanca, Argentine, CERZOS<\/h3>\n

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Universit\u00e9 de P\u00e9rouse (Italie), du 6 septembre 2022 au 8 novembre 2022<\/h3>\n

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L’objectif de ce d\u00e9tachement est d’\u00e9tendre une carte de liaison g\u00e9n\u00e9tique t\u00e9traplo\u00efde d’Eragrostis curvula en utilisant des marqueurs DarT seq et des g\u00e9notypes F1 suppl\u00e9mentaires afin de cartographier plus pr\u00e9cis\u00e9ment la r\u00e9gion impliqu\u00e9e dans l’apomixie. La population F1 a \u00e9t\u00e9 obtenue en croisant un g\u00e9notype sexuel (OTA) et un g\u00e9notype apomictique (Don Walter) comme parent maternel et paternel, respectivement. Cette nouvelle carte de liaison facilitera la localisation des loci contr\u00f4lant l’apomixie (diplosporie) et permettra d’\u00e9tudier l’\u00e9volution du g\u00e9nome de cette gramin\u00e9e (WP3). En collaboration avec le groupe UNIPG, j’analyse les marqueurs mol\u00e9culaires (DarT seq) obtenus chez les nouveaux individus F1 et chez d’autres individus obtenus pr\u00e9c\u00e9demment \u00e0 Bah\u00eda Blanca par notre groupe pour construire une nouvelle carte de liaison int\u00e9gr\u00e9e, ainsi que deux cartes de liaison sp\u00e9cifiques aux parents. Pour cela, une analyse de s\u00e9gr\u00e9gation et la d\u00e9tection de marqueurs SNP polymorphes dans l’un ou les deux parents sont n\u00e9cessaires pour r\u00e9soudre ces trois cartes de liaison. De plus, la reconstruction des haplotypes en utilisant cette population F1 sera explor\u00e9e ainsi que le profil SNP des parents.<\/p>\n

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Davide Perrone<\/h2>\n

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Universit\u00e9 de Milan, Italie<\/h3>\n

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IICAR (Rosario, Argentine), du 2 septembre 2022 au 28 novembre 2022<\/h3>\n

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Davide Perrone (Universit\u00e9 de Milan, Italie) est accueilli au laboratoire du Prof. Silvina Pessino \u00e0 IICAR CONICET, Rosario, Argentine, du 3 septembre au 27 novembre 2022. Son travail, men\u00e9 en collaboration avec le Dr. Carolina Colono (IICAR-CONICET-UNR), vise \u00e0 utiliser la transformation biolistique pour produire des lign\u00e9es fluorescentes de Paspalum notatum DR5 (marqueur d’auxine) et TCS (marqueur de cytokinine) avec diff\u00e9rents modes de reproduction (sexualit\u00e9 et apomixie aposporique). Ces mat\u00e9riels v\u00e9g\u00e9taux seront utiles pour \u00e9tudier les profils hormonaux \u00e0 diff\u00e9rents stades de d\u00e9veloppement de l’ovule chez un mod\u00e8le apomictique bien caract\u00e9ris\u00e9. En outre, Davide contribue \u00e0 l’\u00e9tude du r\u00f4le du g\u00e8ne ARF10 (AUXIN RESPONSE FACTOR 10) dans le transport de l’auxine dans l’ovule d’Arabidopsis, en effectuant des caract\u00e9risations cyto-embryologiques de croisements arf10 x PIN1 et arf10 x DII-Venus obtenus pr\u00e9c\u00e9demment \u00e0 l’IICAR-CONICET-UNR. Enfin, il est impliqu\u00e9 dans l’analyse de la connexion fonctionnelle entre ARF10 et TRIMETHYL GUANOSINE SYNTHASE (TGS1), un g\u00e8ne impliqu\u00e9 dans le contr\u00f4le de l’aposporie chez Paspalum.<\/p>\n

De gauche \u00e0 droite: Carolina Colono, Silvina Pessino, Davide Perrone<\/p>\n

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Sol Vega, doctorante<\/h2>\n

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Rosario, Argentine, IICAR<\/h3>\n

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IRD (France), du 15 juin 2022 au 16 ao\u00fbt 2022<\/h3>\n

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Maria Sol est doctorante \u00e0 l’IICAR-CONICET, Argentine, et elle visite le laboratoire du Dr. Olivier Leblanc \u00e0 l’IRD (Montpellier, France). L’objectif principal de son s\u00e9jour est d’initier l’analyse fonctionnelle de mutants d’Arabidopsis d\u00e9fectueux pour le g\u00e8ne IAA16, dont l’orthologue chez Paspalum notatum<\/em> (PsIAA30) pr\u00e9sente une r\u00e9duction importznte de son expression pendant le d\u00e9veloppement apomictique (Siena et al., Plants 2022, 11, 472). Les mutants iaa16<\/em> h\u00e9t\u00e9rozygotes et homozygotes seront analys\u00e9s par des observations cytoembryologiques afin de rechercher d’\u00e9ventuels ph\u00e9notypes reproducteurs femelles rappelant l’apomixie aposporique. Ce travail contribuera \u00e0 d\u00e9terminer les effets de la d\u00e9pl\u00e9tion de IAA16 sur le d\u00e9veloppement reproductif d’Arabidopsis et \u00e0 planifier une caract\u00e9risation fonctionnelle plus pouss\u00e9e de ce g\u00e8ne ou d’autres g\u00e8nes appartenant \u00e0 la m\u00eame voie. Pendant sa visite, Maria Sol travaillera en collaboration avec le Dr Olivier Leblanc et Caroline Michaud de l’IRD, ainsi qu’avec Lorena Siena, sa directrice de th\u00e8se \u00e0 l’IICAR.<\/p>\n

Lorena Siena (gauche) & Sol Vega (droite)<\/p>\n

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Dr. Juan Pablo A. Ortiz<\/h2>\n

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Rosario, Argentine, IICAR<\/h3>\n

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IRD (France), du 6 juini 2022 au 7 juillet 2022<\/h3>\n

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Un nouveau d\u00e9tachement MAD pour initier la caract\u00e9risation des g\u00e9nomes apomictiques (WP3) a d\u00e9but\u00e9 le 7 juin 2022. \u00c0 cette occasion, le Dr Juan Pablo A. Ortiz de l’IICAR (CONICET-UNR, Argentine) visitera le laboratoire du Dr Olivier Leblanc \u00e0 l’IRD (France). Le plan de travail comprend l’annotation d’un assemblage du g\u00e9nome d’un \u00e9cotype diplo\u00efde de bahiagrass Pensacola (Paspalum notatum, var. saurae Parodi), obtenu pr\u00e9c\u00e9demment dans le cadre d’une collaboration entre des laboratoires fran\u00e7ais, italiens et d’argentin. Nous avons \u00e9galement l’intention d’identifier et de caract\u00e9riser les s\u00e9quences li\u00e9es au locus contr\u00f4lant l’apomixie (ACL) chez une plante t\u00e9traplo\u00efde. En particulier, nous utiliserons la technologie ONT \u00ab\u00a0adaptive sampling\u00a0\u00bb qui permet le s\u00e9quen\u00e7age de r\u00e9gions d’int\u00e9r\u00eat. Nous esp\u00e9rons donc enrichir notre s\u00e9quen\u00e7age avec des lectures sp\u00e9cifiques de la plante apomictique t\u00e9traplo\u00efde et ensuite assembler l’ACL. Olivier Leblanc, C\u00e9dric Mariac et Julie Orjuela (Unit\u00e9 de recherche DIADE), ainsi que Juan Manuel Vega (MAD d\u00e9tach\u00e9 de l’IICAR) contribueront \u00e0 ce travail.<\/p>\n

Juan Pablo Ortiz (gauche) & Olivier Leblanc (droite)<\/p>\n

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Dr. Lorena Siena<\/h2>\n

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Rosario, Argentine, IICAR<\/h3>\n

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IRD (France), du 6 juin 2022 au 1er septembre 2022<\/h3>\n

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Pendant ce d\u00e9tachement de 3 mois, Lorena Siena (IICAR, CONICET- Argentine) visitera l’IRD avec l’objectif de faire progresser la caract\u00e9risation fonctionnelle du g\u00e8ne TGS1 au cours du d\u00e9veloppement reproducteur de la plante mod\u00e8le Arabidopsis thaliana. Les activit\u00e9s seront men\u00e9es en collaboration avec le Dr. Olivier Leblanc et son groupe de l’IRD, et comprennent : des tests de compl\u00e9mentation utilisant la construction pTGS1:TGS1-GUS ; la d\u00e9termination de l’origine des sacs embryonnaires suppl\u00e9mentaires observ\u00e9s chez les mutants tgs1<\/em> en utilisant des lign\u00e9es rapportrices (pWOX2:CENH3:GFGP) \u00e0 la fois de l’identit\u00e9 gam\u00e9tophytique femelle et du nombre de chromosomes (origine r\u00e9duite vs. non r\u00e9duite) , et; l’analyse des donn\u00e9es RNAseq obtenues \u00e0 partir d’embryons pr\u00e9coce (stade globulaire) de type sauvage et mutants tgs1<\/em>. Cette \u00e9tude vise \u00e0 d\u00e9terminer les alt\u00e9rations des profils d’expression g\u00e9nique caus\u00e9es par la d\u00e9pl\u00e9tion de TGS1, \u00e0 identifier les g\u00e8nes cibles potentiels, et \u00e0 explorer si l’\u00e9pissage alternatif contr\u00f4le l’embryogen\u00e8se pr\u00e9coce chez les plantes.<\/p>\n

Olivier Leblanc (gauche) & Lorena Siena (droite)<\/p>\n

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Dra. Luciana Delgado<\/h2>\n

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Rosario, Argentine, IICAR<\/h3>\n

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IRD (France), du 20 mai 2022 au 19 ao\u00fbt 2022<\/h3>\n

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Dans le cadre de ce d\u00e9tachement, le Dr Luciana Delgado (IICAR) et le Dr Daphn\u00e9 Autran travaillent ensemble pour \u00e9tudier la communication entre la division, l’expansion et la diff\u00e9renciation cellulaire tout au long du d\u00e9veloppement reproducteur femelle chez P. rufum, une esp\u00e8ce apomictique. L’objectif global est de construire un atlas 3D du d\u00e9veloppement pr\u00e9coce de l’ovule. Pendant le s\u00e9jour, nous \u00e9tablirons les conditions optimales de clarification des ovules de P. rufum et les techniques de coloration de la paroi cellulaire pour obtenir des images 3D \u00e0 haute r\u00e9solution. Les images seront segment\u00e9es avec MorphoGraphX, class\u00e9es par stade de d\u00e9veloppement, et enfin une analyse quantitative des descripteurs cellulaires sera effectu\u00e9e. Nous pensons que la compr\u00e9hension de la coordination entre les processus de d\u00e9veloppement reproductif avec la croissance et la division cellulaire, dans un contexte apomictique, pourrait \u00eatre \u00e9clairante pour comprendre la r\u00e9gulation de l’apomixie.<\/p>\n

Daphn\u00e9 Autran (gauche) & Luciana Delgado (droite)<\/p>\n

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Dr. Juan Pablo Selva<\/h2>\n

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Bahia Blanca, Argentine, CERZOS<\/h3>\n

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Universit\u00e9 de Milan (Italie), du 16 mai 2022 au 16 juillet 2022<\/h3>\n

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L’objectif de ce d\u00e9tachement est de poursuivre la caract\u00e9risation fonctionnelle d’un g\u00e8ne diff\u00e9rentiellement exprim\u00e9 dans les plantes sexu\u00e9es et apomictiques d’Eragrostis curvula<\/em> en utilisant des mutants de ma\u00efs portant une insertion Mutator dans le g\u00e8ne orthologue.<\/p>\n

Pendant ces deux mois, j’ai collabor\u00e9 avec le groupe du professeur Gabriella Consonni pour \u00e9valuer diff\u00e9rentes populations de ma\u00efs F2 qui ont \u00e9t\u00e9 plant\u00e9es dans un champ exp\u00e9rimental. Les plantes ont \u00e9t\u00e9 g\u00e9notyp\u00e9es en utilisant des amorces sp\u00e9cifiques pour l’insertion du transposon situ\u00e9e dans le premier exon du g\u00e8ne orthologue et des amorces sp\u00e9cifiques pour l’all\u00e8le de type sauvage. Les activit\u00e9s comprenaient \u00e9galement l’observation au microscope de pistils clarifi\u00e9s pour rechercher des anomalies au cours du d\u00e9veloppement reproductif femelle.<\/p>\n

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Dr. Juan Pablo A. Ortiz<\/h2>\n

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Rosario, Argentine, IICAR<\/h3>\n

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IBBR (Italie), du 5 mai 2022 au 6 juin 2022<\/h3>\n

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Un nouveau d\u00e9tachement MAD impliquant des chercheurs d’Argentine et d’Italie commence le 9 mai pour un mois ! En effet, le Dr Juan Pablo A. Ortiz de l’IICAR<\/a>, (CONICET-UNR) est accueuilli au laboratoire du Dr Fulvio Pupilli \u00e0 l‘Institut des Biosciences et des Bioressources<\/a> (IBBR-CNR) de P\u00e9rugia. Le plan de travail est centr\u00e9 sur l’analyse fonctionnelle de ORC3, un g\u00e8ne qui s\u00e9gr\u00e8ge comme compl\u00e8tement li\u00e9 \u00e0 l’apomixie, au cours de la formation de l’albumen de Paspalum<\/em> simplex<\/em> apomicts. En outre, il est \u00e9galement pr\u00e9vu de r\u00e9aliser l’assemblage du g\u00e9nome de P. simplex<\/em> en utilisant les long reads g\u00e9n\u00e9r\u00e9s par la technologie de s\u00e9quen\u00e7age Oxford Nanopore. Les membres du groupe du Dr. Pupilli impliqu\u00e9s dans ces projets sont : Mar\u00eda Eugenia C\u00e1ceres, Andrea Rubini, Francesca De Marchis et Michele Bellucci. A la fin du d\u00e9tachement, nous esp\u00e9rons avoir termin\u00e9 la r\u00e9daction d’un manuscrit sur l’analyse fonctionnelle de PsORC3<\/em> et produit un projet d’assemblage du g\u00e9nome de P. simplex<\/em>.<\/p>\n

Fulvio Pupilli (gauche) & Juan Pablo A. Ortiz (droite)<\/p>\n

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Dr. Carolina Colono & Prof. Silvina Pessino<\/h2>\n

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Rosario, Argentine, IICAR<\/h3>\n

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Universit\u00e9 de Milan (Italie), du 29 avril 2022 au 2 juillet 2022<\/h3>\n

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Du 29 avril au 2 juillet 2022, la Dr. Carolina Colono et la Prof. Silvina Pessino visiteront le laboratoire du Prof. Lucia Colombo \u00e0 l’Universit\u00e9 de Milan, pour explorer le r\u00f4le du contr\u00f4le hormonal dans la transition de la sexualit\u00e9 \u00e0 l’apomixie en utilisant la plante mod\u00e8le Arabidopsis thaliana<\/em>.<\/p>\n

De gauche \u00e0 droite: Carolina Colono, Silvina Pessino et Lucia Colombo<\/p>\n

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Juan Manuel Vega, doctorant<\/h2>\n

[\/et_pb_text][et_pb_text admin_label=\u00a0\u00bbCountry\u00a0\u00bb _builder_version=\u00a0\u00bb4.17.0″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb header_3_font=\u00a0\u00bbInter|300|||||||\u00a0\u00bb header_3_font_size=\u00a0\u00bb16px\u00a0\u00bb header_3_line_height=\u00a0\u00bb24px\u00a0\u00bb custom_margin=\u00a0\u00bb||0px||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb|30px|28px|30px|false|true\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Rosario, Argentine, IICAR<\/h3>\n

[\/et_pb_text][et_pb_text admin_label=\u00a0\u00bbWhere\u00a0\u00bb _builder_version=\u00a0\u00bb4.17.0″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb header_3_font=\u00a0\u00bbInter|300|||||||\u00a0\u00bb header_3_font_size=\u00a0\u00bb16px\u00a0\u00bb header_3_line_height=\u00a0\u00bb24px\u00a0\u00bb custom_margin=\u00a0\u00bb||0px||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb|30px|28px|30px|false|true\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

IRD, Montpellier (France), du 19 mars 2022 au 18 juin 2022<\/h3>\n

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Ce d\u00e9tachement s\u2019int\u00e8gre aux activit\u00e9s du projet MAD visant \u00e0 d\u00e9terminer la nature, l’\u00e9volution et le r\u00f4le fonctionnel de la r\u00e9gion contr\u00f4lant l’aposporie (ACR) chez Paspalum notatum <\/em>(voir objectifs WP3). En utilisant un g\u00e9nome de r\u00e9f\u00e9rence diplo\u00efde et un jeu de s\u00e9quences \u00ab\u00a0long reads\u00bb pour des plantes apomictiques, j’utiliserai la m\u00e9thode \u201cadaptive sampling\u201d propos\u00e9e par la plateforme ONT pour enrichir le s\u00e9quen\u00e7age en lectures longues de l’ACR et ensuite r\u00e9aliser un assemblage \u00e0 haute couverture pour une plante apomictique obligatoire et une autre apomictique facultative. Des analyses compl\u00e9mentaires utilisant des m\u00e9thodes open source pour identifier et qualifier les variations structurelles seront \u00e9galement appliqu\u00e9es.\u00a0 Ces travaux devraient permettre des approches de g\u00e9nomique structurelle et comparative pour d\u00e9terminer le contenu g\u00e9nique et la capacit\u00e9 de codage\/r\u00e9gulation de l’ACR et, de fournir des indices sur son r\u00f4le fonctionnel dans l’\u00e9mergence de l’apomixie chez Paspalum<\/em>.<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00a0\u00bb1_2″ admin_label=\u00a0\u00bbcolumn white\u00a0\u00bb _builder_version=\u00a0\u00bb4.17.0″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb border_radii=\u00a0\u00bbon|8px|8px|8px|8px\u00a0\u00bb box_shadow_style=\u00a0\u00bbpreset4″ box_shadow_horizontal=\u00a0\u00bb0px\u00a0\u00bb box_shadow_blur=\u00a0\u00bb60px\u00a0\u00bb box_shadow_color=\u00a0\u00bbrgba(0,8,54,0.16)\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_image src=\u00a0\u00bbhttps:\/\/apomixis.eu\/wp-content\/uploads\/2022\/04\/Dr-Diego-Zappacosta.png\u00a0\u00bb title_text=\u00a0\u00bbDr-Diego-Zappacosta\u00a0\u00bb _builder_version=\u00a0\u00bb4.16.0″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb custom_margin=\u00a0\u00bb||0px||false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_image][et_pb_text admin_label=\u00a0\u00bbD Zappacosta\u00a0\u00bb _builder_version=\u00a0\u00bb4.16.0″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb header_2_font=\u00a0\u00bbInter|600|||||||\u00a0\u00bb header_2_font_size=\u00a0\u00bb28px\u00a0\u00bb header_2_line_height=\u00a0\u00bb36px\u00a0\u00bb custom_margin=\u00a0\u00bb||0px||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb32px|30px|4px|30px|false|true\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Dr. Diego Zappacosta<\/h2>\n

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Bahia Blanca, Argentine, CERZOS<\/h3>\n

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Universit\u00e9 de P\u00e9rouse (Italie), du 1er mars 2022 au 7 mai 2022<\/h3>\n

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L’objectif de ce d\u00e9tachement est de poursuivre l’assemblage des g\u00e9nomes t\u00e9traplo\u00efdes d’Eragrostis curvula pour d\u00e9terminer le locus contr\u00f4lant l’apomixie et \u00e9tudier l’\u00e9volution des g\u00e9nomes chez cette gramin\u00e9e (WP 3). Pendant ces deux mois, je vais analyser, en collaboration avec le groupe de l’UNIPG, les relations g\u00e9n\u00e9tiques entre le diplo\u00efde sexu\u00e9 (cv Victoria), pr\u00e9c\u00e9demment s\u00e9quenc\u00e9 et publi\u00e9 par les 2 institutions, et le t\u00e9traplo\u00efde apomictique cv. Tanganyika. Nous assemblerons d’abord un pan-g\u00e9nome et le d\u00e9composerons en \u00ab core genome \u00bb et \u00ab dispensable genome \u00bb . Les s\u00e9quences qui correspondent \u00e0 la r\u00e9f\u00e9rence Victoria seront assembl\u00e9es tandis que les autres seront assembl\u00e9es de novo pour analyser les variations de type pr\u00e9sence\/absence. Nous analyserons ensuite les g\u00e8nes orthologues pour trouver les contractions et les expansions des familles de g\u00e8nes et comparerons les r\u00e9gions contr\u00f4lant l’apomixie chez Eragrostis et Paspalum spp. Finalement, j’appliquerai le protocole MCSeEd d\u00e9velopp\u00e9 par l’UNIPG pour comparer les r\u00e9gions diff\u00e9rentiellement m\u00e9thyl\u00e9es entre les g\u00e9notypes apomictiques et sexuels (Task 3.3).<\/p>\n

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Personnel d\u00e9tach\u00e9La p\u00e9riode de d\u00e9tachement est la p\u00e9riode pass\u00e9e par un membre du projet dans un institut h\u00e9bergeant, aux fins du projet. La personne d\u00e9tach\u00e9e est le membre h\u00e9berg\u00e9.En raison […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","inline_featured_image":false,"_price":"","_stock":"","_tribe_ticket_header":"","_tribe_default_ticket_provider":"","_tribe_ticket_capacity":"0","_ticket_start_date":"","_ticket_end_date":"","_tribe_ticket_show_description":"","_tribe_ticket_show_not_going":false,"_tribe_ticket_use_global_stock":"","_tribe_ticket_global_stock_level":"","_global_stock_mode":"","_global_stock_cap":"","_tribe_rsvp_for_event":"","_tribe_ticket_going_count":"","_tribe_ticket_not_going_count":"","_tribe_tickets_list":[],"_tribe_ticket_has_attendee_info_fields":false},"ticketed":false,"_links":{"self":[{"href":"https:\/\/apomixis.eu\/fr\/wp-json\/wp\/v2\/pages\/838"}],"collection":[{"href":"https:\/\/apomixis.eu\/fr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/apomixis.eu\/fr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/apomixis.eu\/fr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/apomixis.eu\/fr\/wp-json\/wp\/v2\/comments?post=838"}],"version-history":[{"count":147,"href":"https:\/\/apomixis.eu\/fr\/wp-json\/wp\/v2\/pages\/838\/revisions"}],"predecessor-version":[{"id":3850,"href":"https:\/\/apomixis.eu\/fr\/wp-json\/wp\/v2\/pages\/838\/revisions\/3850"}],"wp:attachment":[{"href":"https:\/\/apomixis.eu\/fr\/wp-json\/wp\/v2\/media?parent=838"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}